The subset of B lymphocytes having IgG on their surfaces was purified from human spleen and blood using a fluorescence-activated cell sorter (FACS). This subset constituted about 15% of B lymphocytes. The remaining non-IgG-bearing B cells were also obtained for study. These two populations were examined for (a) their expression of other surface immunoglobulin isotypes, (b) their ability to give...

Outbred Wistar rats immunized with native type II collagen developed ear lesions resembling those of human relapsing chondritis. As in human disease, these lesions were characterized by intense chondritis, positive immunofluorescence reactions to IgG and C3, and circulating IgG reactive with native type II collagen. Furthermore, electron-dense deposits were seen near the surface of chondrocytes...

The aqueous two-phase system (ATPS) with polyethylene glycol (PEG) and hydroxypropyl starch (HPS) was evaluated for the extraction of human immunoglobulin G (IgG) from human serum albumin (HSA). The partitions of IgG in PEG/sulphate, PEG/phosphate, PEG/Dextran and PEG/HPS ATPSs were compared, and the results indicated that PEG/HPS system was most suitable for IgG extraction. The effects of the ...