نتایج جستجو برای: hsa denaturation

تعداد نتایج: 10128  

2011
Yonghua Han Jiahao Chen Xiaokun Zhao Chaozhao Liang Yong Wang Liang Sun Zhimao Jiang Zhongfu Zhang Ruilin Yang Jing Chen Zesong Li Aifa Tang Xianxin Li Jiongxian Ye Zhichen Guan Yaoting Gui Zhiming Cai

BACKGROUND MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing. METHODOLOGY/PRINCIPAL FINDINGS We detected 656 differentially expressed known human miRNAs and miRNA antisense sequences (miRNA*s)...

Journal: :The Journal of Cell Biology 1993
C Nathan Q W Xie L Halbwachs-Mecarelli W W Jin

Spreading of neutrophils on protein-coated surfaces is a pivotal event in their ability to respond to soluble, physiologic agonists by releasing large amounts of hydrolases and oxidants. Using neutrophils plated on serum-, fibrinogen- or fibronectin-coated surfaces, we investigated the effect of human serum albumin (HSA) on spreading-dependent neutrophil responses. HSA suppressed the respirator...

2007
Yun Mo Micheal E. Barnett Dolores Takemoto Harriet Davidson Uday B. Kompella

PURPOSE To assess the potential of human serum albumin nanoparticles (HSA NP) as a nonviral vector for ocular delivery of Cu, Zn superoxide dismutase (SOD1) gene. METHODS Cu, Zn superoxide dismutase (SOD1) gene-encapsulated nanoparticles (NP) were developed using human serum albumin (HSA), an endogenous protein, by a desolvation-crosslinking method. The pSOD-loaded HSA NP was evaluated for in...

Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...

2014
Francisco Dominguez Juan Manuel Moreno-Moya Teresa Lozoya Ainhoa Romero Sebastian Martínez Mercedes Monterde Marta Gurrea Blanca Ferri Maria Jose Núñez Carlos Simón Antonio Pellicer

Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by rea...

Journal: :Analytical and bioanalytical chemistry 2011
Ryan Matsuda Jeanethe Anguizola K S Joseph David S Hage

This study used high-performance affinity chromatography (HPAC) to examine the binding of gliclazide (i.e., a sulfonylurea drug used to treat diabetes) with the protein human serum albumin (HSA) at various stages of modification due to glycation. Frontal analysis conducted with small HPAC columns was first used to estimate the number of binding sites and association equilibrium constants (K(a))...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2010
Iria Vázquez Miren Maicas Nerea Marcotegui Ana Conchillo Elisabet Guruceaga José Roman-Gomez María J Calasanz Xabier Agirre Felipe Prosper María D Odero

We read with great interest the work published by Dickstein et al. (1) showing that induced EVI1 expression in a murine model silences miR-124 expression by DNA methylation. The EVI1 gene codes for a transcription factor implicated in the development and progression of high-risk acute myeloid leukemia (AML) (2, 3). We quantify the expression of 250 microRNAs (miRNAs; TaqManHuman miRNA assay set...

Journal: :Drug metabolism and pharmacokinetics 2004
Daisuke Harada Makoto Anraku Hikaru Fukuda Shinsaku Naito Kumiko Harada Ayaka Suenaga Masaki Otagiri

The binding properties of the disulfide covalent bond between N-acetyl-L-cysteine (NAC) and human serum albumin (HSA) were investigated. HSA, purified from either healthy subjects or renal failure patients, was incubated with NAC in buffer and analyzed by 4VP-EG-Me column chromatography, which can distinguish between the redox states of the only free thiol of HSA. Although intact HSA was found ...

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