Mitoxantrone is a marker substrate of breast cancer resistance protein (BCRP). BCRP involved in number pharmacokinetic drug-drug interactions. The transporter’s possible saturability makes it advisable to use low concentrations mitoxantrone for vitro studies. Consequently, quantification requires method with high sensitivity. aim the study was develop and validate procedure Caco-2 culture media...

A reliable solid-phase extraction – high-performance liquid chromatography (SPE-HPLC) method for the determination of flavonoids in European Bupleurum species B. baldense Turra and B. affine Sadler was developed. Extraction of aerial parts by ultrasound with 80% aqueous methanol and fractionation of flavonoids by means of SPE allowed a good recovery of analytes between 99 and 104%. The subseque...

Sirolimus (rapamycin) has a macrolide structure and is under clinical investigation as an immunosuppressant after organ transplantation. An HPLC/mass spectrometry assay to quantify sirolimus in blood was developed. 28-O-Acetyl sirolimus was used as internal standard. Blood samples were extracted with C18 columns. The extracts were injected into an HPLC system and isocratically eluted with metha...

1.1 Materials ........................................................................................................................ 2 1.2 Instrumentation .............................................................................................................. 2 1.3 HPLC and mass spectrometry ........................................................................................ 3 1.4 C...

In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...

This work describes a simple, rapid, and reliable HPLC method for the determination of organic acids in fermented shrimp waste. Lactic, acetic and citric acids were quantified by HPLC with UV detection, on a 250 ́4.6 mm Extrasil ODS 5-mm column, mobile phase was ultrapure water adjusted with metaphosphoric acid to pH=2.1, flow rate 0.6 mL/min, column temperature 30 °C, and detection wavelength 2...

Absolute quantification of peptides is typically achieved using amino acid analysis, elemental analysis or derivatisation chemistry. Impurities, if present, may be accounted for using analytical high-performance liquid chromatography (HPLC) with detection of the peptide bond ultraviolet (UV) absorbance. To do this, peak areas from a UV chromatogram are used to estimate percentage purity on a ma...

Hydrochloric acid extraction of feces in the presence of ether yields an extract suitable for spectrophotometric estimation of total porphyrin and for further separation by "high-performance" liquid chromatography (HPLC) or thin-layer chromatography. A total porphyrin reference interval of less than 200 nmol/g dry weight of feces was established from data on 106 normal subjects on an unrestrict...