BACKGROUND/AIMS Combination therapy utilizing tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) in conjunction with other anticancer agents, is a promising strategy to overcome TRAIL resistance in malignant cells. Recently, parthenolide (PT) has proved to be a promising anticancer agent, and several studies have explored its use in combination therapy. Here, we investigated ...

In mature erythrocytes, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) yield NADPH, a crucial cofactor of the enzyme glutathione reductase (GR) converting glutathione disulfide (GSSG) into its reduced state (GSH). GSH is essential for detoxification processes in and survival of erythrocytes. We explored whether the anti-inflammatory compounds Bay 11-7082,...

Microtubules are centrally involved in cell division, being the principal components of mitotic spindle. Tubulin, the constituent of microtubules, can be cyclically modified on its alpha-subunit by enzymatic removal of the COOH-terminal tyrosine residue by an ill-defined tubulin carboxypeptidase (TCP) and its readdition by tubulin tyrosine ligase (TTL). We and others have previously shown that ...

It is observed that some venom may have an anticancer effect. Hemiscorpius lepturus is a most dangerous scorpion in Iran. Heminecrolysin is the most toxic fraction of Hemiscorpius lepturus venom and it is responsible for hemolytic effects of the venom. In this research, we purified heminecrolysin by FPLC, and HPLC methods and used MTT assay in prostatic cancer cell line (PC3 cells). We observed...

PURPOSE To compare clinical and radiographic results of anterior cruciate ligament (ACL) reconstruction using modified transtibial technique (mTT), anteromedial (AM) portal technique and outside-in (OI) technique. MATERIALS AND METHODS From March 2007 to December 2012, ACL reconstruction was performed using the mTT, AM portal technique and OI technique in 20 patients each. Anteroposterior and...

An ethanolic extract of Allium tuberosum Rottl. demonstrated potent choline acetytrasferase (ChAT) activating activity (45%) among 30 screened Korean edible plants. The ChAT activator of A. tuberoum Rottl. was purified and identified as ferulic acid [4-hydroxy-3-methoxycinnamic acid] via silica-gel open-column chromatography, HPLC, EI-MS, and 1H/13C-NMR. The ferulic acid from A. tuberoum Rottl....

In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts used to assess bioactivities plant. The antimicrobial activity was tested against a panel Gram-positive -negative pathogenic bacterial fungal strains using microdilution method. cytotoxicity K. investigated human-derived macrophage THP-1 cells through MTT assay....

Abstract Rubus hyrcanus Juz. (Rosaceae), known as Caspian blackberry, is wildly distributed around the Sea. This study focused on antioxidant, cytotoxic, and antibacterial activities of total extracts different fractions from roots leaves this species. The phenolics flavonoid contents were also evaluated. Finally, phenolic profiles selected determined using HPLC–DAD LC–MS/MS. results indicated ...

We describe a test which uses the ability of viable cells to reduce 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to detect resistance to a bactericidal drug, rifampin, in in vitro-cultured Mycobacterium tuberculosis. The assay shows a linear relationship between the number of viable bacteria and the ability to reduce MTT. Dead mycobacteria were unable to reduce MTT. Rifam...

An in-depth investigation of a multi-mode reconfigurable aperture controlled by pin diode switches is presented. A method for reducing the coupling across the pin diode switches is explored, yielding a noticeable improvement in the isolation characteristics between ports. Strategic feeding of the patch antenna ports results in significantly improved radiation patterns, making the system suitabl...