1. A preparation of the specific polysaccharide of Type VIII pneumococcus is described in which the use of heat, strong acid, and alkali were avoided. 2. Quantitative estimations are given of the homologous and cross reacting precipitin and agglutinin in Types III and VIII antisera produced in the rabbit and in the horse. Quantitative data are also given on the mechanism of the Type VIII precip...

Different physical factors like aeration, temperature and shaking culture conditions were applied to the culture and observation of growth was studied for 72 hours of incubation. Similarly the Mycoplasma mycoides subspecies capri was grown in PPLO medium enriched with different concentrations (5, 10 and 20%) of serum from horse, bovine and allantoic fluid, along with usual concentration of yeas...

1. Within 24 hours following intravenous inoculation with rabbit virulent strains of pneumococcus Type III, most rabbits develop infections of one or both knees. The frequency of bilateral knee joint involvement increases as the duration of the disease is prolonged. 2. The spinal fluid, aqueous humor, and bladder urine remain sterile at a time when the knee joints contain pneumococci. Subsequen...

A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. A modified HL-1 medium supplemented with either horse or bovine serum was used. Cultures were initiated in a humidified atmosphere containing 2% O2, 5% CO2 and 93% N2 at 37...

For efficient production of cultured meat, chicken breast muscle cells and leg were proliferated differentiated using medium with various serums, proliferation differentiation ability analyzed. Chicken serum-free (NC), 20% FBS (C), 10% serum (CS) 5% horse (HS) (T1), CS (T2), HS (T3). Using the immunofluorescence staining, Pax7 nuclei stained in to confirm that they satellite cells. As a result ...

This report investigates the regulation of DNA synthesis in trophoblast stem cells and differentiating trophoblast cells. Experiments in this study were performed on the Rcho-1 trophoblast cell line, which was established from a transplantable rat choriocarcinoma. Rcho-1 trophoblast cells can be manipulated to proliferate or differentiate along the trophoblast giant cell pathway. DNA synthesis ...

A therapeutic anti-rabies immunoglobulin for human use has been produced mainly in horses. The presently available seroneutralization test, the rapid fluorescent focus inhibition test (RFFIT), is laborious and rather difficult to carry out in horse farms. This study was undertaken to develop a simple latex agglutination test (LAT) for determining rabies antibodies in horse sera. LAT was validat...

A plaque-forming agent arose spontaneously during cloning of Acholeplasma oculi 19L. The agent produced plaques on A. oculi 19L and A. oculi-i, but not on A. laidlawii, A. modicum, or wild isolates of A. oculi. The agent required horse serum for plaque formation as well as for adsorption to the indicator lawn; however, it was extremely sensitive to an inhibitor in some horse sera. The agent ret...

Adoption of nongenetic offspring occurs in a variety of species but is rare in equids. We report a case of adoption by a free-ranging, feral mare Equus caballus and compare the maternal care received by her genetic offspring (born 1995) to that of her adopted offspring (born 1996) for the first 30 weeks of development. We compare five measures of care: (1) total time spent suckling, (2) mare ag...