The routine study of bone marrow trephine biopsies involves fixation, decalcification, paraffin-embedment, sectioning and staining. However, this process creates artifacts, produces shrinkage of tissue, consumes time and can result in sections of unsatisfactory cytological quality. It also renders the tissue unsuitable for enzyme-histochemical and immunohistochemical analyses. Frozen section of...

The acroblast of Helix spermatids is a duplex structure: it consists of an 'externum' (which appears dark by positive phase-contrast microscopy and becomes impregnated with osmium) enclosing a region called the 'internum'. Histochemical studies show the externum to be composed principally of phospholipid and to contain glycolipid, alkaline phosphatase, and an enzyme which splits thiamine pyroph...

AKAGUCHI'S test for arginine depends on the production of a pink or red colour when certain guanidine derivatives are treated with a-naphthol and sodium hypochlorite in alkaline solution (Sakaguchi, 1925). This test had been familiar to biochemists for 19 years before it was independently adapted to histochemical use in the same year by Serra (1944) and myself (1944). Our results were published...

Immunofluorescence and a new histochemical technique were employed to assay 226 breast cancer specimens for estrogen receptor. Results showed an overall correlation of 91 percent when compared to those of biochemical assays. The histochemical technique is rapid, easy to perform and reveals the same parameters as does immunofluorescence without the need for antiserum. Tumor cell receptor heterog...

The distribution of aluminium has been investigated using a new histochemical technique in transiliac bone biopsy specimens from 20 patients with aluminium related bone disease and 17 with other metabolic bone diseases. The method, which employs solochrome azurine at acid pH (acid solochrome azurine), showed a wider distribution of aluminium within cortical and trabecular bone than other histoc...

A novel technique combining the freeze drying and embedding in glycol methacrylate at low temperature of tissue permitted the histochemical demonstration of a variety of enzymes, showing maintenance of enzyme activity, accurate enzyme localisation without apparent diffusion, and excellent morphological detail. The results obtained with this new approach were superior to standard techniques used...

ECENT ADVANCES in histochemical techR niques have stimulated interest in the inicroscopic differentiation of various mucopolysaccharides. Following a previous studyLo on the analysis of mucoid substances in normal tissue, the present paper deals with the application of some of the newer methods to the examination of pathological material. For this purpose, three types of niucus-contaiiiing tumo...

Tissue obtained from 55 men with prostatic disease was assayed for estrogen and androgen receptors by a newly developed histochemical technique. The material studied consisted of 45 specimens of benign nodular prostatic hyperplasia and 10 specimens of prostatic adenocarcinoma. The results obtained were compared to those of parallel biochemical assays in 17 cases and successfully correlated in 8...

Background Sheifer et al indicated that undetected infarction account for at least 20% of all infarctions and carry a prognosis as poor as detected ones [1]. Large myocardial infarction has been measured using both 3D and 2D delayed enhancement MRI, which was compared with histochemical staining [2]. The purpose of this MRI study was to 1) measure diffuse and large infarction size using delayed...

Introduction . Cytomegalovirus infection (CMVI) makes a significant contribution to the development of pregnancy pathologies, including dysfunction placenta. However, mechanism such an effect CMV has not been precisely established. For coordination biochemical reactions in placenta, optimal concentration substances, nucleotides, is required. Nucleoside diphosphatase enzyme that catalyzes hydrol...