‎In addition to his countless contributions to spectral graph theory‎, some works of Dragos Cvetkovic are concerned with chemical problems‎. These are briefly outlined‎, ‎with emphasis on his collaboration with‎ ‎the present author‎.

abstract the oldest comic playwright from ancient world, whose works have survived and reached us, is aristophanes the greek. names of many comic playwrights are found during the 5th century b.c. in ancient greece; however, works of none of whom is survived virtually complete. thus, the only criterion for judging comedies of golden age of ancient greece is applied to the works of aristophanes. ...

pushkin is an extraordinary phenomenon in russian and also the world literature with works on which the global ideas shine in. nobody is able, just like pushkin, to demonstrate a man of other nations and to speak on behalf of him so authentically.  his ability in describing iranian culture and also iranian genius poet, saadi, was so that prince shalikov, the publisher of “damskii zhurnal”, call...

Conrad’s acclaimed works from his middle period have been thoroughly studied from several perspectives including postcolonialism whereas the novels from his early period were overlooked due to their so-called“uneven” quality. The most notable works among Conrad’s early novels are hisLingard Trilogy- three of his early novels which are based on the recurring presence of the Captain Tom Lingard, ...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...