The kinetic properties of the nonmitochondrial ATP-dependent Ca sequestering mechanism in disrupted nerve terminal (synaptosome) preparations have been investigated with radioactive tracer techniques; all solutions contained DNP, NaN3, and oligomycin, to block mitochondrial Ca uptake. The apparent half-saturation constant, KCa, for the nonmitochondrial Ca uptake is approximately 0.4 micrometer ...

BACKGROUND We performed a comparative study on Ca2+ release activity of the sarcoplasmic reticulum and calcium sensitivity of contractile apparatus of chemically skinned myocardial fibers obtained from four nonfailing human hearts and 13 excised hearts from patients with idiopathic dilated cardiomyopathy. METHODS AND RESULTS Ca2+ sensitivity of contractile apparatus was studied by following t...

OBJECTIVE Annexin A5 is a phosphatidylserine binding protein that binds dying cells in vivo. Annexin A5 is a potential molecular imaging agent to determine efficacy of anti-cancer therapy in patients. Its rapid clearance from circulation limits tumor uptake and, hence, its sensitivity. The aim of this study is to determine if non-invasive imaging of cell death in tumors will benefit from increa...

cAMP-activated Na+ current (INa,cAMP) was studied in voltage-clamped neurons of the seaslug Pleurobranchaea californica. The current response to injected cAMP varied in both time course and amplitude as the tip of an intracellular injection electrode was moved from the periphery to the center of the neuron soma. The latency from injection to peak response was dependent on the amount of cAMP inj...

S. Xu, H. Zhu, M. Styner, W. Gao, V. Jewells, D. Shen, and W. Lin Computer Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, Biostatistics, University of North Carolina at Chapel Hill, United States, Psychiatry, University of North Carolina at Chapel Hill, United States, Radiology, University of North Carolina at Chapel Hill, United States, Neuroradiology, Un...

Background:   To assess the outcome of the remplissage arthroscopic surgical method in patients with anterior shoulder dislocation associated with Hill-Sachs lesion.     Methods:   Ten patients with anterior shoulder dislocations and Hill-Sachs lesions were entered into this study and were operated on by the remplissage arthroscopic surgical method. They were followed up 22 months after surgery...

From the *School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, Division of Sports Medicine, Campus Heath Services, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, AliveCor Corporation, San Francisco, California, and xDivision of Cardiology, Cardiac Electrophysiology, University of North Carolina at Chapel Hill, Chapel Hill, Nort...

Proanthocyanidin is a phenolic compound present in plants, that has antioxidant, antinociceptive, anti-emetic, and neuroprotective properties. We investigated the actions of proanthocyanidin from grape seeds on 5-hydroxytryptamine (5-HT)(3) receptors in NCB-20 neuroblastoma cells using a whole-cell voltage clamp technique. Co-treatment of proanthocyanidin (0.3-100 µg/ml) and 3 µM 5-HT (near EC(...