Preparative HPLC 5}30 '20 2}20 0.01}1 High purity solvent required Higher resolution See also: II/Chromatography: Countercurrent Chromatography and High-Speed Countercurrent Chromatography: Instrumentation. Chromatography: Liquid: Countercurrent Liquid Chromatography. III/Alkaloids: Gas Chromatography; High-Speed Countercurrent Chromatography; Liquid Chromatography; Thin-Layer (Planar) Chromato...

The influence of mobile phase composition on the retention of selected test analytes in different normal- and reversed-phase chromatographic systems is studied. A novel adsorption model for an accurate prediction of the analyte retention in the column chromatography with binary mobile phase is proposed. Performance of the model is compared with the retention model reported in the literature. Bo...

Six components of new cephem antibiotics, cephabacin M1-6, were isolated from the culture filtrate of Xanthomonas lactamgena YK-431 by various types of column chromatographies and preparative reverse-phase HPLC. Their structures were determined by spectroscopic analyses and degradation studies. They consist of 7-methoxydeacetylcephalosporin C as a nucleus and a tri- to heptapeptide including a ...

A simple automatic analytical system, consisting of separate modules, for liquid chromatography has been constructed. The different parts of the automatic machine are an auto sampler, an auto dispenser, a selector valve with eight channels, a heater/cooler, a mixing chamber and a pressure air driven injector valve. The process was controlled by a PC from an easily changeable run protocol. The s...

A new high-performance liquid chromatography (HPLC) method has been developed to detect ultra-low concentrations of bisphenol-A (BPA) (below 1 ng/L (ppt)) using column switching electrochemical detection (ECD). The results were superior to those obtained from manual pretreatment procedure with membrane stationary phase. BPA is inherently ubiquitous in the environment, including tools and solven...