نتایج جستجو برای: high cell density cultivation
تعداد نتایج: 3765094 فیلتر نتایج به سال:
در این تحقیق خواص فیزیکی و مکانیکی چندسازه چوب پلاستیک حاصل از ترکیب های مختلف پلاستیک بازیافتی به همراه آرد چوب مورد بررسی قرار گرفت. نسبت پلاستیک های بازیافتی بر طبق نسبت پلاستیک ها در زباله های جامد شهری تهران انتخاب شد. برای این منظور چندسازه چوب پلاستیک با ماتریس یک نوع پلاستیک بازیافتی(high density polyethylene)، مخلوط دو hdpe/ low density polyethylene)، مخلوط سه (hdpe/ ldpe/ polypropylen...
Background For industrial pharmaceutical protein production fast growing, high producing and robust cell lines are required. To select more pH shift permissive and fast growing sub-populations, the CHO DP-12 (ATCC clone #1934) cell line, an anti-IL8 antibody producing CHO K1 (DHFR) clone, was continuously subcultured at high viability (>90 %) for more than four hundred days in shaking flasks us...
Microbially produced fatty acids are potential precursors to high-energy-density biofuels, including alkanes and alkyl ethyl esters, by either catalytic conversion of free fatty acids (FFAs) or enzymatic conversion of acyl-acyl carrier protein or acyl-coenzyme A intermediates. Metabolic engineering efforts aimed at overproducing FFAs in Escherichia coli have achieved less than 30% of the maximu...
For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior system for suspension cultures of mammalian cells based on orbital shaker technology. We found that "square-shaped" bottles (square bottles) ...
Recombinant Streptococcus Protein G (PG) is a cell wall protein, which, when combined with mammal immunoglobulin, is used in separating antibody technology. High-density fermentation technologies using an engineered recombinant PG-producing bacteria as well as PG separation and purification technologies have a direct impact on the availability and application of PG. Through primary and secondar...
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