Endophytic fungi associated with rhizomes of four cultivars of Zingiber officinale were identified by molecular and morphological methods and evaluated for their activity against soft rot pathogen Pythium myriotylum and clinical pathogens. The volatile bioactive metabolites produced by these isolates were identified by GC-MS analysis of the fungal crude extracts. Understanding of the metabolite...

A phosphate-solubilizing bacterial strain designated OK2(T) was isolated from rhizospheric soil of grasses growing spontaneously in a soil from Spain. Cells of the strain were Gram-negative, strictly aerobic, rod-shaped and motile. Phylogenetic analysis of the 16S rRNA gene indicated that this bacterium belongs to the gamma-subclass of Proteobacteria within the genus Pseudomonas and that the cl...

A protocol for metabolic profiling of mice urine was developed based on gas chromatograph-mass spectrometer (GC-MS) to explore metabolic state directly. The intra-day, inter-day, repeatability, and stability RSD for most endogenous compounds were less than 3%. Type 2 diabetic mellitus (T2DM) mice model was induced by high calorie diet combined with streptozocin. Urine from the control, T2DM and...

Background: Since chemical anticancer drugs are found to have adverse effect on body, nowadays people motivated towards natural agents treat cancer. Fruits and vegetables potential source of that protect our body against cancer by scavenging neutralizing free radicals. Objective: The study was aimed explore antioxidant properties methanolic extract leaves Hibiscus sabdariffa L. (MEHSL). Methods...

Sleep symptoms are associated with weight gain and cardiometabolic disease. The potential role of diet has been largely unexplored. Data from the 2007-2008 National Health and Nutrition Examination Survey (NHANES) were used (n = 4552) to determine which nutrients were associated with sleep symptoms in a nationally representative sample. Survey items assessed difficulty falling asleep, sleep mai...

The present study was aimed to examine the phytochemical constituents present in Andrographis paniculata (Burm.f.) Wall. ex Nees. The preliminary phytochemical constituents were qualitatively analyzed using the method described by Brindha et al. For GC-MS analysis, 10 g powdered sample is extracted with 30 mL ethanol overnight and filtered in ashless filter paper with sodium sulphate (2 g) and ...

The mechanism of action of isoniazid (INH), a first-line antituberculosis drug, is complex, as mutations in at least five different genes (katG, inhA, ahpC, kasA, and ndh) have been found to correlate with isoniazid resistance. Despite this complexity, a preponderance of evidence implicates inhA, which codes for an enoyl-acyl carrier protein reductase of the fatty acid synthase II (FASII), as t...

The present study aims to optimize the processing variables producing liquid smoke from mabang wood (Shorea pachyphylla) by using Response Surface Methodology (RSM). In this investigation, a design of experiment with different combinations pyrolysis temperature and time on yield was applied. response optimal yield, temperature, predicted mathematical model. operating conditions for process yiel...

Plants are excellent sources of secondary metabolites that have been used for the treatment human diseases. The plant Momordica cymbalaria contains different types phytochemicals like phenols, steroids, flavonoids, alkaloids and tannins. present investigation was carried out to determine bioactive compounds in parts by Gas chromatography- Mass spectroscopy (GC-MS) technique. Chromatograms vivo ...

Bouea macrophylla Griff is a member of the Anacardiaceae family, which has edible fruits. Due to high nutritional value, appealing taste, and attractive color, fruit juice B. potential be developed as functional beverage. The present study was conducted determine phytochemical profile using GC-MS LC-MS/MS. analysis performed with Agilent Technologies 7890 Gas Chromatograph automated sampling ta...