PURPOSE This study investigated the connection among HER-2 gene amplification, HER-2 protein expression, and markers of tumor angiogenesis and oxygenation in patients with operable, invasive breast tumors. EXPERIMENTAL DESIGN From 1988 to 1995, 425 patients with metastatic breast cancer were enrolled in a study of high-dose chemotherapy with autologous transplant. Primary tumor blocks were ob...

abstract is not available.

materials and methods in the first stage, no2 pollutant concentrations were measured using the data collected from air pollution monitoring stations. sampling was performed in six stations. the chosen stations have been based on the us environmental protection agency (epa) standards. in the next step, the data were processed using excel software and information was given to the airq model. data...

Congenital insensitivity to pain with anhidrosis is a rare disease of the nervous system which causes one to lose his/her feeling of pain. The disease is subtype four of hereditary sensory and autonomic neuropathy (HSAN IV) that results from NTRK1 gene defect. Direct sequencing was performed to screen NTRK1 for mutations. The result revealed a homozygous deletion of adenine on intron 14 that ma...

BACKGROUND Linear regression of efficiency (LRE) introduced a new paradigm for real-time qPCR that enables large-scale absolute quantification by eliminating the need for standard curves. Developed through the application of sigmoidal mathematics to SYBR Green I-based assays, target quantity is derived directly from fluorescence readings within the central region of an amplification profile. Ho...

objective her-2/neu gene is overexpressed in diverse human cancers and studies suggest a role of its product – p185 protein – in tumor progression by specifically promoting the invasive capacity of tumor cells. our aim was to evaluate her-2/neu content in resectable gastric cancer in this geographical region and assess the relationship between p185 expression and clinicopathologic tumor paramet...

The combination of chromatin immunoprecipitation with next-generation sequencing technology (ChIP-seq) is a powerful and increasingly popular method for mapping protein-DNA interactions in a genome-wide fashion. The conventional way of analyzing this data is to identify sequencing peaks along the chromosomes that are significantly higher than the read background. For histone modifications and o...

introduction: oxygen tension is one of the most important stimuli in stem cell biology. in this study, we investigate the considerable influence of hypoxia on cx43 gene expression as one of the most important gap junction on the surface of mesenchymal stem cells. methods: mesenchymal stem cells were isolated from c57bl/6 mouse bone marrow and cultured in dmem medium under low oxygen tension (1%...

PURPOSE To assess the effect of chromosome 17 copy number on HER-2/neu status determination in breast cancers. EXPERIMENTAL DESIGN HER-2/neu gene copy and chromosome 17 centromere numbers were evaluated on 893 breast carcinomas using double color fluorescence in situ hybridization (FISH). The net and chromosome 17 corrected (ratio) HER-2/neu copy numbers were compared and related to immunohis...