More than 90% of the Plesiomonas shigelloides strains that we tested produced a beta-hemolysin, as judged by the results of agar overlay and contact-dependent hemolysis assays. The hemolysin was cell associated, was active against the erythrocytes of various animal species, and was synthesized at both 25 and 35 degrees C. Activity was lost after thermal or proteolytic treatments or after preinc...

In this study, a number of 85 Staphylococcus aureus isolates were collected from various clinical sources including diabetic foot, burns, urine, wound, abcesses, boils, nasal discharge and vaginal smear from 135 inpatients at different Mansoura hospitals, Dakahlia governorate, Egypt. Isolates were phenotypically investigated for some virulence factors (biofilm, lipase, protease, staphylokinase ...

BACKGROUND Quorum sensing is a mechanism of intercellular communication that controls the production of virulence factors in Pseudomonas aeruginosa. Inhibition of quorum sensing can disarm the virulence factors without exerting stress on bacterial growth that leads to emergence of antibiotic resistance. OBJECTIVES Finding a new quorum sensing inhibitor and determining its inhibitory activitie...

Background: Candida glabrata is the second agent of candiduria with increased resistance to antifungals. Microsatellite length polymorphism (MLP) one genotyping techniques used in epidemiological investigation improve clinical management. Objectives: We aimed detect different genotypes C. isolates using six microsatellite markers and MLP technique. Moreover, our genotypes' association other cou...

OBJECTIVE To investigate the virulence factors including hemolysin production, β-lactamase production, and biofilm formation. Antimicrobial resistance and plasmid content of 20 Escherichia coli isolates obtained from feces and Tigris water were screened. METHODS Ten clinical and ten environmental E. coli isolates were collected from children diarrhea and swim areas on Tigris River in Baghdad ...

Pathogenic microorganisms are persistently expressing resistance towards present generation antibiotics and are on the verge of joining the superbug family. Recent studies revealed that, notorious pathogens such as Salmonella typhi, Shigella dysenteriae and Vibrio cholerae have acquired multiple drug resistance and the treatment became a serious concern. This necessitates an alternative therape...

A Vibrio cholerae cytotoxin, designated VcVac, was found to cause vacuolation in Vero cells. It was originally detected in the pathogenic O1 Amazonia variant of V. cholerae and later shown to be produced in environmental strains and some El Tor strains. Comparison of VcVac production in various strains suggested that hemolysin was responsible for the vacuolating phenotype. Genetic experiments e...

The enterotoxicity of an El Tor-like hemolysin purified from non-O1 Vibrio cholerae was investigated. Fluid accumulation was induced by injection of purified hemolysin into the ligated intestinal loops in adult rabbits (De test), intraintestinal administration in infant rabbits (Dutta test), and oral inoculation in suckling mice. The accumulated fluid was invariably mucous and bloody, and a his...

Materials and methods: Overall 45 isolates (20 Candida albicans and 25 non-albicans Candida spp.) genotyped by repPCR were included in this study. Virulence factors were studied in both aerobic and anaerobic conditions. In isolates, egg yolk agar was used for determining phospholipase activity, while bovine serum albumin agar was used for proteinase activity, Tween-80 medium for esterase activi...

Three classes of immunologically reactive cells, differing only slightly in size from each other, are required for the production of hemolysin-forming cells in culture. The three classes of cells can be detected in the normal mouse spleen by the combined use of rosette formation, velocity sedimentation, and irradiation. One class of cells (peak sedimentation velocity, 3.2 mm per hr) forms roset...