The protective effects of propolis on biochemical and hematological parameters were appraised in the blood of Oncorhynchus mykiss exposed to cypermethrin (CYP). The fish were subjected to three sublethal concentrations of CYP (0.0041, 0.0082 and 0.0123 ppm) and propolis (10 ppm) for 96 h. The samples were analysed to changes in the biochemical parameters, such as the metabolits: glucose, total ...

By T. H. J. HUISMAN, A. M. Dozy, B. E. HORTON AND J. B. WILsON T HE EXISTENCE of variants of normal human fetal hemoglobin ( HbFor a2y2 ) has been suggested in different reports in the literature. Variants of fetal hemoglobin with abnormal a-polypeptide chains have been described5 ”5 and some are well characterized.’0 Only one abnormality, namely the electrophoretically fast moving hemoglobin v...

It is hypothesized that oxidative reactions of hemoglobin driven by reactive oxygen species in the vasculature lead to endothelial cell injury or death. Bovine aortic endothelial cells were incubated with diaspirin cross-linked hemoglobin (DBBF-Hb), developed as a hemoglobin-based oxygen carrier, and hydrogen peroxide (H(2)O(2)), generated by the glucose oxidase system. The low steady flux of H...

The efficiency of hemoglobin as an oxygencarrying substance is dependent upon certain features of its molecular configuration. The functional integrity of the molecule depends not only on spatial relationships between the heme and globin moieties, but on interrelations between the four globin chains (1, 2). The functional significance of portions of the molecule can be investigated by a study o...

INTRODUCTION. Helicobacter pylori infection in gastric mucosa may cause systemic inflammatory reaction. We investigated the inflammatory effect of H pylori infection on nutritional factors such as serum albumin in hemodialysis patients and influence of eradication of H pylori on this association. MATERIALS AND METHODS. Ninety-eight patients on hemodialysis were divided into 2 groups according t...

Enthalpies and entropies have been determined for the reversible binding of O2 and CO to chelated protoheme, a compound having a covalently attached imidazole bound to the iron. The values, based upon 1 atm standard state, are delta HO2 = -14.0 kcal (1 kcal = 4.18 kJ)/mol, delta SO2 = -35 eu, delta HCO = -17.5 kcal/mol, delta SCO = -34 eu, delta H identical to O2 = 21 kcal/mol (dissociation), a...

Background—Nitrite can be converted to nitric oxide (NO) by a number of different biochemical pathways. In newborn lambs, an aerosol of inhaled nitrite has been found to reduce pulmonary blood pressure, possibly acting via conversion to NO by reaction with intraerythrocytic deoxyhemoglobin. If so, the vasodilating effects of nitrite would be attenuated by free hemoglobin in plasma that would ra...

Hemoglobin is a classical model allosteric protein. Research on hemoglobin parallels the development of key cooperativity and allostery concepts, such as the 'all-or-none' Hill formalism, the stepwise Adair binding formulation and the concerted Monod-Wymann-Changuex (MWC) allosteric model. While it is clear that the MWC model adequately describes the cooperative binding of oxygen to hemoglobin,...

OBJECTIVE To find out host gene expression profiles after malarial infection. METHODS Further time-course microarray analysis of peripheral blood mononuclear cells focusing on malaria pathogenesis was performed. RESULTS Up-regulation of coagulation-related genes, heat shock proteins, glycolytic enzymes, glucose transporters, and vacuolar H(+)-ATPases was found in acute febrile malaria. In e...