نتایج جستجو برای: hemi nested pcr

تعداد نتایج: 202584  

ژورنال: :پژوهش های آسیب شناسی زیستی 0
shahab falahi m.sc. student, department of medical virology, faculty of medical science, studental research committee, tarbiat modares university, tehran, iran mehrdad ravanshad assistant professor, department of medical virology, faculty of medical science, tarbiat modares university, tehran, iran azra kenar koohi m.sc. student, department of medical virology, faculty of medical science, studental research committee, tarbiat modares university, tehran, iran

هدف: واکنش زنجیر پلیمراز یکی از مهم‏ترین پیشرفت‏ها در زمینه زیست‏شناسی و تشخیص مولکولی است. با وجود سادگی در مفهوم این روش نیازمند کنش‏های پیچیده بین توالی هدف، آغازگرها، dntp و آنزیم پلیمراز به‏منظور تکثیر و تشخیص موفق است. شناسایی ویروس‏های rna دار نیازمند استفاده از روش‏هایی با حساسیت و ویژگی بالا است، بنابراین تکثیر براساس روش nested pcr تا حدی منجر به بهبود حساسیت شده و از سوی دیگر، یک مرح...

Journal: :The Journal of general virology 2001
L González M García-Goti C Cousens P Dewar N Cortabarría A B Extramiana A Ortín M De Las Heras J M Sharp

Peripheral blood leukocytes (PBLs) and tissue samples from 36 sheep were examined for jaagsiekte sheep retrovirus (JSRV) by hemi-nested PCR. Animals were classified according to the status of sheep pulmonary adenomatosis (SPA), which was confirmed by pathological examination, as follows: (i) sheep with classical SPA (cSPA, n=10), (ii) sheep with atypical SPA (aSPA, n=6), (iii) non-affected shee...

Journal: :Revista de saude publica 2008
Julianna Dias Zeidler Pablo Oscar Amézaga Acosta Priscila Pereira Barrêto Joel da Silva Cordeiro

OBJECTIVE To detect the presence of dengue virus in larval forms of Aedes aegypti and to associate vector presence with rainfall and incidence of disease. METHODS Eighteen households were randomly selected for egg collection in a neighborhood of the city of Boa Vista, Roraima, in Northern Brazil. Two oviposition traps were installed per home, and removed after one week. This was repeated on a...

Journal: :BMC Infectious Diseases 2005
Marnix Van Loock Kristel Verminnen Trudy O Messmer Guido Volckaert Bruno M Goddeeris Daisy Vanrompay

BACKGROUND Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp. psittaci outer membrane protein A (ompA) gene in pharyngeal swabs. METHODS The fluorescein-biotin labelled PCR products were immobilized on streptavidin-coated microtiter plates...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2003
Donna J Johnson Eileen N Ostlund Beverly J Schmitt

A traditional nested reverse transcription-polymerase chain reaction (RT-PCR) assay specific for eastern equine encephalomyelitis (EEE) virus was designed to multiplex with a previously described West Nile (WN) virus nested RT-PCR assay. Differentiation of EEE and WN was based on base pair size of the amplified product. One hundred fifty-seven mammalian and avian brain tissues were tested by EE...

Journal: :Journal of clinical microbiology 2003
Manfred Weidmann Ursula Meyer-König Frank T Hufert

The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HS...

Journal: :Acta bio-medica : Atenei Parmensis 2006
Adriana Calderaro Giovanna Piccolo Chiara Gorrini Simona Peruzzi Laura Zerbini Simona Bommezzadri Giuseppe Dettori Carla Chezzi

BACKGROUND AND AIM OF THE WORK In recent years, the diagnosis of toxoplasmosis has been improved by Real-time PCR assays. In this study we compared the performances of two Real-time PCRs (FRET and TaqMan protocols) already described in the literature, and one nested-PCR, currently used in our laboratory for the molecular diagnosis of toxoplasmosis. METHODS We evaluated the sensitivity and the...

Journal: :The European respiratory journal 2010
J S Park Y A Kang S Y Kwon H I Yoon J-H Chung C-T Lee J H Lee

Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB. Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies, followed by nested TB PCR on formalin-fixed pa...

Journal: :jundishapur journal of microbiology 0
reza taherkhani infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of microbiology and parasitology, faculty of medicine, bushehr university of medical sciences, bushehr, ir iran fatemeh farshadpour department of microbiology and parasitology, faculty of medicine, bushehr university of medical sciences, bushehr, ir iran manoochehr makvandi infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9166181683, fax: +98-6113738313 mojtaba hamidifard infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran mahdi esmailizadeh department of medical physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran bijan ahmadi internal medicine department, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions in this study, high prevalence of 91.7% hcmv gb1 genotype was predominant among hcmv-positive uc patients, which suggests that there might be an association between hcmv gb genotype 1 and uc disease. results among 98 patients with uc, only 12 (12.2%) patients were positive for hcmv genome, while the hcmv genome was not detected in any of the controls. (p = 0.002). the distribution o...

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