cd34 is a type i membrane protein with a molecular mass of approximately 110 kda. this antigen is associated with human hematopoietic progenitor cells and is a differentiation stage-specific leukocyte antigen. in this study we have generated and characterized monoclonal antibodies (mabs) directed against a cd34 marker. mice were immunized with two keyhole lympet hemocyanin (klh)-conjugated cd34...

Hematopoietic cell generation in the midgestation mouse embryo occurs through the natural transdifferentiation of temporally and spatially restricted set of hemogenic endothelial cells. These cells take on hematopoietic fate in the aorta, vitelline and umbilical arteries and appear as hematopoietic cell clusters that emerge from the vascular wall. Genetic and live imaging data have supported th...

two basic and clinical researches accomplished during the recent years on embryonic and adult stem cells constituted a mutation in regenerative therapy. these cells can be used for treating some degenerative diseases. between them, age-related functional defects, hematopoietic and immune system disorders, heart failures, chronic liver injuries, diabetes, parkinson’s and alzheimer’s diseases, ar...

Solid tumors are composed of cancerous cells and non-cancerous stroma. A better understanding of the tumor stroma could lead to new therapeutic applications. However, the exact compositions and functions of the tumor stroma are still largely unknown. Here, using a Lewis lung carcinoma implantation mouse model, we examined the hematopoietic compartments in tumor stroma and tumor-bearing mice. Di...

Background and Aims : Background: Atherosclerosis is a chronic inflammatory disease with macrophages as the most abundant hematopoietic cells present in plaques. Resident-like (res-like) were identified healthy atherosclerotic aorta, however, what their role lesional microenvironment not completely understood.

stem cells are self-renewing and undifferentiated cell types that can be differentiate into functional cells. stem cells can be classified into two main types based on their source of origin: embryonic and adult stem cells. stem cells also classified based on the range of differentiation potentials into totipotent, pluripotent, multipotent, and unipotent. multipotent stem cells have the ability...

BACKGROUND Although mobilization of hematopoietic stem cells and hematopoietic progenitor cells can be achieved with a combination of granulocyte colony-stimulating factor and plerixafor (AMD3100), improving approaches for hematopoietic progenitor cell mobilization is clinically important. DESIGN AND METHODS Heparan sulfate proteoglycans are ubiquitous macromolecules associated with the extra...

Numerous transcription factors allow hematopoietic cells to respond to lineage- and stage-specific cytokines and/or to act as their effectors. The transcription factors PU.1 and c-Myb are essential for hematopoiesis, most likely acting at distinct stages of differentiation, but sharing a common set of target genes. To determine whether PU.1 and c-Myb are functionally interrelated, murine bone m...

In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow cytometric analysis showed that CD45(+) c-Kit(+) hematopoietic cells were more abundant in muscle...

Anthracycline antibiotics are potent anticancer drugs widely used in the treatment of solidtumors and hematological malignancies. Because of their extensive clinical use and their toxiceffect on normal cells, in the present study the effect of these drugs on multipotent hematopoieticbone marrow cells was investigated employing, viability tests, PARP cleavage, Hoechst 33258staining, DNA fragment...