Serotype C and D of Clostridium botulinum produce botulinum toxin complex (TC), which is comprised of botulinum neurotoxin, nontoxic nonhemagglutinin, and hemagglutinins (HAs). The TC is capable of aggregating equine erythrocytes via interaction between one of the HAs, namely HA-33, and sugar chains on the cell surface. This hemagglutination is inhibited by specific sugars. In this data article...

Secretory immunoglobulin A (sIgA) antibodies are the first line of defence at the genital mucosa, and are thought to hinder viral infections by binding to conformational epitopes on the viral capsid. To investigate if cervical sIgA binds to conformational epitopes of the Human papillomavirus type 16 (HPV16), cervical mucus samples from 109 HPV16-infected patients were examined in a HPV16 virus-...

The immune adherence hemagglutination assay was found to be as sensitive and specific as the indirect immunofluorescence technique for titration of antibodies to Epstein-Barr virus capsid antigen. Satisfactory virus capsid antigen-specific and negative control antigens for the immune adherence hemagglutination assay were prepared from cell extracts of the Epstein-Barr virus producer P3HR-1 and ...

Indirect hemagglutination test for detection of antibodies to cytomegalovirus is highly sensitive and reproducible, if employed in well-defined conditions. Standardization of the various factors involved is necessary as well as their reciprocal equilibrium : sheep erythrocytes, antigen, dilution of tanin, buffers quality. The hemagglutination test can be performed on small volumes such as blood...

Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adherence hemagglutination test was found to be as specific as the differential abso...

BACKGROUND: Sensitive and specific diagnostic tools are very central to decide on individual case management and at all stages of control programs in schistosomiasis. This study was undertaken to assess the diagnostic efficacy of a commercial indirect hemagglutination test in detection of schistosomiasis mansoni in relation to the Kato method. METHODS: In a cross sectional study undertaken in M...

The Middlebrook-Dubos hemagglutination test with normal red cells and the Boyden hemagglutination test with red cells tanned with tannic acid were shown to be strictly specific, for tubercle polysaccharide and for the protein antigens. The former test detects polysaccharide antibodies and the latter protein antibodies. Convincing evidence is given that polysaccharide does not adhere to the surf...

Serial dilution with Takatsy loops resulted in exaggerated passive hemagglutination titers with most of the anti-bovine serum albumin sera tested. It appears that certain types of agglutinins adhere to the loop surface and are released only gradually. This adherence, or carry-over effect, was prevented by presoaking loops in gelatin or gelatin-rabbit serum-albumin solutions. Hemolysins did not ...

The antigenic properties of the VH region of immunoglobulin heavy chains were studied by means of a fragment corresponding to the variable part of the heavy chain of an IgG3 myeloma protein (KUP) and an antiserum made against this fragment. By hemagglutination, hemagglutination inhibition, and immunofluorescence techniques, it was shown that the anti-VH antiserum detected three sets of antigens...

Through the use of acetone and ether extraction of brain tissue from newborn mice infected with certain arthropod-borne viruses, it has been possible to demonstrate hemagglutinins for chick erythrocytes associated with the following viruses: dengue Type 1, dengue Type 2, Eastern equine encephalitis, Ilhéus, Japanese B, Ntaya, St. Louis, Sindbis, Uganda S, Venezuelan equine encephalitis, West Ni...