نتایج جستجو برای: hek 293t
تعداد نتایج: 4682 فیلتر نتایج به سال:
Human immunodeficiency virus (HIV) infection is the major problem in the world. Nowadays, new anti-retroviral drugs are extracted from medicinal plants, make an important role to supersede synthetic drugs with different side effects. In this study, we studied the Salix aegyptiaca L extract, Iranian herb, as an anti-HIV drug with anti-retroviral effects. We used a single cycle replicable (SCR) H...
Background & aims: Hemophilia B is caused by either functional deficiency or lack of the human coagulation factor IX (hFIX). The current protein-based therapy with plasma-derived proteins increases, the risk of blood-borne pathogens transmission. Therefore, replacement therapy with recombinant hFIX (rhFIX) is an attractive alternative to plasma derived hFIX concentrates. In order to express and...
background: gp41 of hiv (human immunodeficiency virus) is a protein that mediates fusion between viral and cellular membranes. the agent, t-20, which has been approved for hiv inhibition, can restrain gp41 function in the fusion process; nevertheless, it has disadvantages like instability, high cost of production and injection form to be delivered twice a day. methods: several molecules like nb...
لنتی ویروسها از جمله موثرترین ویروسهای نوترکیب برای انتقال ژن به سلولها و بافتهای پستانداران بشمار میرود. در این مطالعه، عملکرد لنتی ویروسهای انسانی را در انتقال ژن خارجی به سلولهای طیور بررسی کردیم. سه ناقل لنتی ویروسی بنامهای ناقل انتقال (ناقل ترانسفر حامل ژن گزارشگر gfp)، ناقل بسته بندی و ناقل غشائی را همزمان به سلولهای مولد ویروس (رده hek-293t) منتقل کردیم. سوپرناتان سلولهای ترانسفکت شده را...
4 Abstract: At physiological oxygen concentrations ([O2]) hypoxia-inducible factor-1α (HIF-1α) is constantly hydroxylated and thus prepared for proteosomal degradation through the action of the prolyl hydroxylases (PHDs) (Jiang et al., 1996). In hypoxia, however, the oxygen-sensitive PHDs are inhibited and HIF-1α is stabilised. Other agents, including cytokines and growth factors have been show...
هدف: هدف از این مطالعه بررسی کارائی جذب DNA و انتقال ژن به سلولهای کبدی جوجه نژاد لگهورن در دو سطح ترانسفکشن و ترانسدوکشن با لنتی ویروسهای نوترکیب و شدت بیان ترانسژن میباشد. مواد و روشها: برای ترانسفکشن یا ترانسدوکشن ویروسی، سلولها در پلیتهای 96 خانه کشت داده شدند و در مراحل مختلف با میکروسکوپ فلورسنس مشاهده گردیدند. تعداد سلولهای GFP (Green Fluorescent Protein-positive) با نرم...
Background: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. Methods: HEK 293T...
Introduction: Glycoprotein B (gB) is the major antigen for induction of humoral responses against human cytomegalovirus (HCMV) making it an attractive candidate for immune prophylaxis. In the present study, the humoral immune response of BALB/c mice to a truncated HCMV gB protein fused with GFP was evaluated. Methods: The truncated gB coding sequence was synthesized and cloned in pEGFPN1 eukary...
Background: HTLV-1 (Human T_cell lymphotropic virus type), with about 20 million individuals infected worldwide, is a global health problem and is endemic in certain area such as Japan and Khorasan province of Iran. HTLV-1 is the causative agent of progressive diseases, Adult T cell Leukemia (ATL), and HTLV-I Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) which have no ...
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