BACKGROUND Efficient induction of fetal hemoglobin (HbF) is considered as an effective therapeutic approach in beta thalassemia. HbF inducer agents can induce the expression of γ-globin gene and produce high levels of HbF via different epigenetic and molecular mechanisms. Thalidomide and sodium butyrate are known as HbF inducer drugs. MATERIAL AND METHODS CD133(+) stem cells were isolated fro...

Mechanisms underlying fetal hemoglobin (HbF) reactivation in stress erythropoiesis have not been fully elucidated. We suggested that a key role is played by kit ligand (KL). Because glucocorticoids (GCs) mediate stress erythropoiesis, we explored their capacity to potentiate the stimulatory effect of KL on HbF reactivation, as evaluated in unilineage erythropoietic culture of purified adult pro...

El Festival Internacional de Cine Rotterdam (IFFR, por sus siglas en inglés) y su fondo, Hubert Bals Fund (HBF), han sido el sello distintivo un conjunto películas que marcaron punto inflexión la producción cinematográfica regional nacional. Con objetivo mapear contexto transnacional las uruguayas beneficiadas este artículo traza recorrido histórico IFFR HBF establece cuáles son marcas identita...

Dr. C. A. Blau, University of Washington, School of Medicine, Division of Medical Genetics, RG-25, Seattle, WA 98195 (USA) Dear Sir, In the recent letter by Salvati et al. [1], no induction of fetal hemoglobin (HbF) was observed in patients with chronic renal failure treated with erythropoietin (epo). We would like to propose that HbF induction was not observed for at least 3 reasons. First, re...

A cytokine-screening assay of cultured peripheral blood cells obtained using immune rosetting and separation of progenitors was developed to identify determinants of fetal hemoglobin (HbF) modulation during adult erythropoiesis. Among the 12 erythroid growth-promoting cytokines tested, stem cell factor (SCF) at a concentration of 50 ng/mL resulted in the most significant increase in cell prolif...

We report in this paper that the DNA-binding drug mithramycin is a potent inducer of gamma-globin mRNA accumulation and fetal hemoglobin (HbF) production in erythroid cells from healthy human subjects and beta-thalassemia patients. Erythroid precursors derived from peripheral blood were grown in 2-phase liquid culture. In this procedure, early erythroid progenitors proliferate and differentiate...

Preeclampsia is one of the most serious pregnancy-related diseases and clinically manifests as hypertension and proteinuria after 20 gestational weeks. The worldwide prevalence is 3-8% of pregnancies, making it the most common cause of maternal and fetal morbidity and mortality. Preeclampsia lacks an effective therapy, and the only "cure" is delivery. We have previously shown that increased syn...

Gilbert's syndrome (GS) is an autosomal inherited disorder characterized by relative deficiency of glucuronyl transferase and poor uptake of unconjugated bilirubin by hepatocytes. Cardiac surgery on cardiopulmonary bypass (CPB) in these patients triggers further hepatic dysfunction. Transesophageal echocardiography (TEE) and Doppler assessment of hepatic vein help in assessing hepatic blood flo...

correlated positively and negatively with the percentage of normal and abnormal cells, respectively (Figure 1B-C). Fluorescent labeling of HbF allowed us to discriminate nonF-cells (Figure 1Di-ii) fromF-cells (Figure 1Diii-iv), and analyze their shape as captured in the brightfield images. The images confirmed the prediction that some RBCs with detectable HbF content still sickle (Figure 1Div),...

The haemoglobins of Xenopus laevis have been studied by carboxymethyl-cellulose column chromatography and by polyacrylamide gel disk electrophoresis. In Xenopus tadpoles, 2 haemoglobins are found (Xenopus-HbFx and Xenopus-HbF^). Both haemoglobins persist throughout tadpole life: Xenopus-HbF\ is the main tadpole haemoglobin; Xenopus-HbFt is present in rather smaller amounts. The proportion of Xe...