introduction: both initiation and maintenance of spermatogenesis are hormonally regulated by fsh and testosterone. in this study, the effects of fsh and testosterone on the in vitro maturation of fresh and frozen- thawed round spermatids were determind. material and methods: cell suspension was isolated from testis of nmri male mice (8-10 weaks old) and divided into two groups .the first part w...

Galeolaria gemineoa is a sessile broadcast-spawning marine invertebrate, whose spermatozoa have been regarded as a sensitive indicator for water quality monitoring. In this study, 10 steps of spermiogenesis have been identified at the ultrastructural level and this differentiation process has been recapitulated in vitro up to the point of spermiogenesis (step 7-9 spermatids). On completion of t...

ninety barley doubled haploid lines originated from crossing between six barley cultivars in cereal research department, seed and plant improvement institute, karaj, iran were evaluated for resistance to powdery mildew and some agronomic traits in field in 2007. six parent cultivars and a powdery mildew susceptible check cultivar, afzal were also included. sixty two doubled haploid lines showed...

Polyclonal antisera raised against polypeptide components of antigenically related rat Sertoli cell secretory protein S70 and S45-S35 heterodimeric protein were used to identify antigenic sites in rat testis during spermatogenesis. Immunofluorescence, immunoperoxidase and immunogold electron-microscopic methods have shown that all antisera recognize immunoreactive sites in the acrosome of devel...

this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

The three-dimensional structure of the Sertoli cell in the miniature "shiba" goat was examined using scanning electron microscopy. In the basal portion of the seminiferous epithelium, spermatogonia and/or spermatocytes were located in compartments enclosed by adjacent Sertoli cells. From the basal aspect, they were situated in successive recesses. In the middle portion, early round spermatids h...

The birthrate following round spermatid injection (ROSI) remains low in current and evidence suggests that factors in the germinal vesicle (GV) cytoplasm and certain substances in the GV such as the nucleolus might be responsible for genomic reprogramming and embryonic development. However, little is known whether the reprogramming factors in GV oocyte cytoplasm and/or nucleolus in GV are benef...

Although the mouse protamine 1 gene (mP1) is first transcribed in round spermatids, its mRNA is not translated until about 1 week later in elongating spermatids. To determine what mP1 sequences are important for its transcriptional and translational regulation, we have constructed fusions between mP1 and the human growth hormone (hGH) structural gene and analyzed their expression in transgenic ...