Root extracts of Rauwolfia serpentina have been used for centuries in Ayurvedic medicine as a panacea for a wide variety of physical as well as mental disorders. The potential of Agrobacterium rhizogenes-mediated genetic transformation for the synthesis of phytomolecules of high pharmaceutical value is now well established and documented. Transgenic roots were induced from R. serpentina leaf ex...

Culture studies of peripheral blood mononuclear cells from 7 entirely typical cases of hairy cell leukemia showed that after culture in the presence of PHA for 2--5 days, the predominant cell type changed from E- SIg+ CIg+ gamma FcR+ muFcR+ hairy cells to an E+ SIg- CIg- gamma FcR-muFcR- population of transformed cells derived from hairy cells. Depletion and readdition experiments demonstrated ...

Abstract The prerequisite for shoot, root or somatic embryo formation in plant in vitro culture is the development of meristem from dedifferentiated cells of the explant tissue. Auxin and cytokinin levels and their relative ratios play a decisive role in inducing the morphogenetic pathways leading to shoot, root or somatic embryo formation in plant in vitro cultures. Exogenous auxin is required...

Recombinant subunit vaccines consisting of the F1 and V antigens of Yersinia pestis have shown remarkable promise for protection of animals and humans against bubonic and pneumonic plague. Previously we demonstrated that intranasal administration of a recombinant protein consisting of the ricin B chain (RTB) fused with an antigen purified from transgenic Nicotiana tabacum (tobacco) hairy root c...

The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was u...

This study was performed to investigate the effect of Ag+ as an elicitor on the activity of antioxidant enzymes, content of H2O2, accumulation of total tocopherol and antioxidant activity in hairy root cultures of Silybum marianum (L.) Gaertn. The hairy roots were treated with 2 mM Ag+. The treated and non-treated hairy roots were harvested in the same time and data on H2O2 content, DPPH, tocop...

Abstract Hairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model analyzing effect various compounds on metabolism. In contrast to many other types in vitro cultures, hairy roots do not require external supply phytohormones medium. Consequently, growth regulators such as auxins cytokinins rarely used elicitors culture...

Resveratrol and its derivatives are natural stilbenes associated with many health benefits that include those conferred by their antioxidant and anticancer properties. While stilbenes can be recovered as an extract from a selected number of plants, these products are not suitable for many applications in the food/pharmaceutical sectors due to high levels of impurities as well as the overall low...

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures...

Hairy root cultures were established after inoculation of Genista tinctoria in vitro shoots with Agrobacterium rhizogenes, strain ATCC 15834. In transformed roots of G. tinctoria grown in Schenk-Hildebrandt medium without growth regulators the biosynthesis of isoflavones, derivatives of genistein and daidzein, and flavones, derivatives of luteolin and apigenin, characteristic for the intact pla...