introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC) and nuclear maturation in a culture system containing pyruvate. Ovaries were obtained from a slaughterhouse and transported to the laboratory within 2 h at 35-39 degrees...

The aim of the present study was to determine whether mesenchymal stem cell-conditioned medium (MSC-CM) modulates apoptotic and stress-related gene expression, and ameliorates maturation and developmental potential of immature human oocytes after artificial activation. A total of 247 surplus immature germinal vesicle (GV) oocytes obtained from infertile women were allocated into two in vitro ma...

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cu...

: The poor in vitro development of pig oocytes and embryos has been blamed on oxidative stress. We sought to find out if combinations Trolox (T), a synthetic cell-permeable derivative vitamin E, ascorbic acid (AA) could improve the maturation rates cultured oocytes. Pig underwent for 44–45 h medium M 199 supplemented with 0 μM T + AA, 100 250 300 750 AA or AA. These were chosen based previous r...

the purpose of this study was to evaluate the use of glass capillary micropipette (gcm) as a vessel forvitrification of bovine oocytes. cumulus-oocyte complexes (cocs) were obtained from slaughter-house andwashed 5 to 6 times in the washing medium (tcm-199 + 20% fbs) and randomly assigned to treatment andcontrol group. in the first step of vitrification, cocs were exposed to first vitrification...

The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1-10 h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to ...

The object of this study was to assess functional maturation in vitro by obtaining data on the fertilization and embryonic competence of human oocytes with or without exposure to meiosis activating sterol (MAS) during maturation in vitro. Immature oocytes were either collected from unstimulated patients with polycystic ovaries (PCO) during gynaecological surgery, or were donated by patients und...