Superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and the comet assay (SCGE) were used as biomarkers to evaluate the oxidative stress and genotoxicity of toluene, ethylbenzene and xylene in earthworms (Eisenia fetida). The results indicated that the exposure of the three pollutants caused a stress response of the three enzymes, an approximate bell-shaped change (a tendency ...

The antifungal efficacy of seed extracts (propanol, ethanol, methanol and sterile deionised water) of Camelina sativa cv. Calena (EC643910) against Rhizopus stolonifer was investigated by agar disc diffusion method. Among all extracts, methanolic seed extract (Disc 3) showed significant activity against R. stolonifer. However, no activity was observed against rest of the extracts (Disc 1, 2 and...

To investigate the effects of Cd on tolerance and antioxidant activities of castor, two different castor (Ricinus communis L.) cultivars (Zibo No. 5 and Zibo No. 8) were used for a hydroponic experiment (0, 1 and 2mg/L Cd) and a pot experiment using Cd-contaminated soil (34mg/kg) with the addition of ethylenedinitrilotetraacetic acid (EDTA). The results indicated that there were significant dif...

abstract pythium aphanidermatum is one of the most important agent of root and foot rot of cucumber, particularly in the warm humid climate. induction of host resistance by microorganisms or by the synthetic compounds is recently considered as one of the biological control method of disease. trichoderma harzianum bi is a good candidate for induction of resistance in plant against diseases. the ...

The ability to engineer proteins by directed evolution requires functional expression of the target polypeptide in a recombinant host suitable for construction and screening libraries of enzyme variants. Bacteria and yeast are preferred, but eukaryotic proteins often fail to express in active form in these cells. We have attempted to resolve this problem by identifying mutations in the target g...

The open reading frame alr1585 of Anabaena sp. strain PCC 7120 encodes a heme-dependent peroxidase (Anabaena peroxidase [AnaPX]) belonging to the novel DyP-type peroxidase family (EC 1.11.1.X). We cloned and heterologously expressed the active form of the enzyme in Escherichia coli. The purified enzyme was a 53-kDa tetrameric protein with a pI of 3.68, a low pH optima (pH 4.0), and an optimum r...

The electrochemical oxidation of guaiacol (2-methoxyphenol) on a treated disk of gold was studied by cyclic voltammetry. At low concentration, guaiacol undergoes one irreversible anodic peak . The current of this peak has a diffusional origin. For guaiacol concentrations higher than 4 . 10 mol dm, the voltammogram shows a post-peak. The effect of experimental data on the ratio of these two peak...

ABSTRACT. Organic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based material (HbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL-1 of hemoglobin) obtaining a size 9-10 µm. The encapsulation percentage weight yield HbNFs@Cu were determined as 100%...

The normal P.D. across the protoplasm of Valonia macrophysa is about 10 mv. negative (inwardly directed). On adding 0.01 M guaiacol to the sea water the P.D. becomes positive and then slowly returns approximately to the normal value. In many cases this behavior is not much affected by raising the pH and so increasing the concentration of the guaiacol ion but in other cases such an increase make...

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxid...