A simple, rapid and validated HPLC method was developed for determination of Piracetam in film coated tablets. A Lichrosorb® (RP-18 column with a mobile phase consisting of acetonitrile – phosphate buffer (5:95v/v)) was used. Quantitative evaluation was performed at 205 nm. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical ...

Ursolic acid is a triterpenoid compound in the plant kingdom and has important biological functions. In this study, ursolic contents of nine herbs including Anethum graveolens (dill), Camellia sinensis L. (green tea), Lepidium sativum (garden cress), Ocimum basilicum (sweet basil), Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), Salvia (sage), Thymus vulgaris (thyme) Urtica d...

Design and development of analytical methods with environment friendly reagents solvents is the need hour for labs engaged in services. Every year thousands chemical pharmaceutical laboratories worldwide are generating tons toxic wastage causing environmental pollution thereby endangering human existence. The objective this study was to develop validate a sensitive, stability-indicating, accura...

Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...

abstract        background and objective: measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. the aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (rp-hplc) with pre-column derivatization by o-phthalaldehyde (opa) in combination with 3- mercaptopropionic...

High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities. This review covers the importance of R...

Relative hydrophobicity and lipophilicity of 63 compounds with known permeability through the blood-brain barrier (BBB) was examined by partitioning in aqueous dextran-poly(ethylene glycol) two-phase system and octanol-buffer system, and by gradient RP-HPLC at pH 7.4. Combination of the relative hydrophobicity estimates, N(CH(2)) obtained by aqueous two-phase partitioning and the lipophilicity ...

The previously reported (Partridge et al., Nature 247, 391-392, 1974 ) glycosylation of the capsid proteins of cowpea mosaic virus (CPMV) has been reinvestigated. In initial studies, a preparation of purified CPMV particles was hydrolysed with HCl and amino acids and sugars were derivatized with o-phthalaldehyde (OPA). No glucosamine or galactosamine, amino sugars previously reported to occur i...

Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250 mm×4.6 mm, 5 mm) using a mobile phase of 10 mM phosph...

A simple highly sensitive, rapid, precise, accurate and specific RP-HPLC method was developed for quantification of eflornithine hydrochloride (2-difluoromethyl-DL-ornithine; DFMO) in its commercial formulation. In this RP-HPLC method, the separation was performed using BDS Hypersil 5μ C18 (150 X 4.6 mm) column at room temperature by using methanol: 2% of Glacial acetic acid in water (80:20 v/v...