AIM To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. METHODS AND RESULTS DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpo...

The 16S rDNA polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and sequencing method has been demonstrated to be valuable in detecting pathogens in the blood of patients suffering from fever or neutropenia. However, its use in the diagnosis of neonatal late‑onset septicemia (LOS) has not yet been reported. The aim of the present study was to investigate the efficien...

Prediction programs for general PCR (or random PCR) products had been developed [3, 2]. These programs had been experimentally shown to be highly accurate in its prediction [2]. On the other hand, random PCR have been demonstrating that it is very useful as an elementary technology of genome profiling [1], in which random PCR products (a set of DNA fragments extracted from various parts of geno...

BACKGROUND To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis. MATERIALS AND METHODS Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects...