Antibodies raised against an intracellular and extracellular domain of the GH secretagogue receptor (GHS-R) confirmed that its topological orientation in the lipid bilayer is as predicted for G protein-coupled receptors with seven transmembrane domains. A strategy for mapping the agonist-binding site of the human GHS-R was conceived based on our understanding of ligand binding in biogenic amine...

OBJECTIVE The present study investigated the effects of different dosages of a GHS-R antagonist [D-Lys3] on some serum hormonal (cortisol, T3 and T4) and biochemical parameters in a rat. MATERIALS AND METHODS Thirty-six 60-day-old male rats were assigned to four treatments. [D-Lys3]-GHRP-6 solutions were infused via intraperitoneal injections. Blood was collected and analyzed. RESULTS The l...

2014 The energy spectrum of a tight-binding honeycomb lattice in the presence of a uniform magnetic field is analysed. The graph of the spectrum over a wide range of rational reduced flux 03A6/03A60 through elementary hexagonal cells is plotted. The energy spectrum is found to have recursive properties similar to those discussed previously on the square and triangular lattices. New features of ...

Hypercalciuria in genetic hypercalciuric stone–forming (GHS) rats is accompanied by intestinal Ca hyperabsorption with normal serum 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] levels, elevation of intestinal, kidney, and bone vitamin D receptor (VDR) content, and greater 1,25(OH) 2 D 3 -induced bone resorption in vitro. To test the hypothesis that hyperresponsiveness of VDR gene expression to 1...

1,3,5-Tris(4-phosphonophenyl)benzene was synthesized via a microwave heating assisted route and was subsequently used for the preparation of a new zirconium phosphonate with honeycomb-like structure displaying remarkable thermal stability and hydrolysis resistance.

The atomic and electronic structures of the Si(111)-E3×E3-Ag surface are studied by first-principles calculations based on the density functional theory. It is found that a structural model consisting of two inequivalent Ag triangles is energetically more favorable than the well-established honeycomb-chained-triangle (HCT) model. The new structure should yield an empty-state STM image with a he...

Synthetic ligands have been identified that reset and amplify the cycle of pulsatile GH secretion by interacting with the orphan GH-secretagogue receptor (GHS-R). The GHS-R is rhodopsin like, but does not obviously belong to any of the established G protein-coupled receptor (GPCR) subfamilies. We recently characterized the closely related orphan family member, GPR38, as the motilin receptor. A ...

A novel G11-protein-coupled receptor specific for synthetic GH-releasing peptides (GHRPs) has recently been cloned and sequenced. Two forms exist, types 1a and 1b, the latter of which is biologically inactive. Using RT-PCR, we looked for the presence in tumorous pituitary cells of messenger ribonucleic acid (mRNA) for this novel GH secretagogue receptor (GHS-R). Both subtypes of GHS-R mRNA were...

Design of the DNA origami structures. Using caDNAno, we designed the following three DNA origami structures: a straight 6by-3 honeycomb-pleated structure (HC), a straight 4-by-4 squarepleated structure (SQ), and a 6-by-3 honeycomb-pleated structure having a 90◦ programmable bend (HC-90◦). Below, we briefly describe our protocols for obtaining the caDNAno designs. Building a DNA origami structur...

Conventional performance evaluation mechanisms focus on dedicated systems. Grid computing infrastructure, on the other hand, is a shared collaborative environment constructed on virtual organizations. Each organization has its own resource management policy and usage pattern. The non-dedicated characteristic of Grid computing prevents the leverage of conventional performance evaluation systems....