Many thiol-containing molecules show heavy metal complexation ability and are used as antidotes. In this study, the potential function associated with thiol-containing peptides (TCPs) from soy protein hydrolysates as natural detoxicants for heavy metals is reported. TCPs enriched by Thiopropyl-Sepharose 6B covalent chromatography had different molecular weight distributions as well as different...

The Kunitz trypsin inhibitor (KTI) and the Bowman-Birk inhibitor (BBI) of trypsin and chymotrypsin contain disulfide bonds. Glycinin, the major storage protein in soybeans also contains disulfide bonds. Treatment of soy white flour with a NADP-thioredoxin system (NTS) effectively reduced disulfide bonds in soy flour and increased protein digestibility by trypsin and pancreatin as measured by th...

Changes of structure and thermal stability of soy protein isolate after pulsed electric field treatment were analyzed by Fourier transform infrared spectroscopy and differential scanning calorimetry (DSC). When the applied pulsed electric field (PEF) treatment intensity was over 35 kV/cm, the amino acid side chain, anti-parallel b-sheets, b-turn as well as b-sheets in soy protein isolate (SPI) ...

BACKGROUND We recently cloned and described a vicilin and showed it to be a major cashew allergen. Additional IgE-reactive cashew peptides of the legumin group and 2S albumin families have also been reported. Here, we attempt to clone, express and characterize a second major cashew allergen. METHODS A cashew cDNA library was screened with human IgE and rabbit IgG anti-cashew extract antisera,...

Soybean is the preferred protein source for both poultry and swine feed. However, this status being challenged due to competition from alternative feed ingredients. To overcome this, it becomes necessary breeders develop soybean cultivars that contain higher better nutritional composition. In study, we have developed experimental lines not only significantly amounts of but also improved sulfur ...

Abstract Bacillus velezensis is widely used for agricultural biocontrol, due to its ability enhance plant growth while suppressing the of microbial pathogens. However, there are few reports on application in fermented feed. Here, a two-stage solid-state fermentation process using followed by Lactobacillus plantarum was developed degrade antinutritional factors (ANFs) and improve soybean meal (S...

Seed oil is important not only for human and animal nutrition, but also for various industrial applications. Numerous genetic engineering strategies have been attempted to increase the oil content per seed, but few of these strategies have involved manipulating the transporters. Pyruvate is a major source of carbon for de novo fatty acid biosynthesis in plastids, and the embryo's demand for pyr...

Large amounts of the major storage proteins, beta-conglycinin and glycinin, in soybean (Glycine max) seeds hinder the isolation and characterization of less abundant seed proteins. We investigated whether isopropanol extraction could facilitate resolution of the low abundant proteins, different from the main storage protein fractions, in one-dimensional polyacrylamide gel electrophoresis (1D-PA...