The levels of a number of enzymes of carbohydrate metabolism were assayed in the cancerous and morphologically normal (host) portions of livers from nine primary malignant hepatoma cases obtained at autopsy. The levels of these enzymes in two normal human adult and two fetal livers were also determined. The activities of phosphoglucomutase, fructose 1,6-diphosphatase and a-glycerophosphate dehy...

Acetylcholine stimulates the exchange of phosphate in phosphatidic acid in guinea pig brain cortex slices (1) and in cytoplasmic particulate fractions of brain which are undergoing oxidative phosphorylation (2). In this paper the reactions involved in the acetylcholine effect in brain microsomal preparations have been investigated. The data presented here indicate that in brain microsomes phosp...

The inhibitory/repressive effect of the final reaction product (inorganic o-phosphate) and the inducing effect of a substrate (β-glycerophosphate) on the phosphatase activity and synthesis in a salt lake sediment were studied. The determinations have been differentially carried out, for acid, neutral and alkaline phosphatase, and in reaction mixtures without buffering. The acid phosphatase acti...

BACKGROUND This study aims to investigate the effects of a modified, balanced crystalloid including phosphate in a perioperative setting in order to maintain a stable electrolyte and acid-base homeostasis in the patient. METHODS/DESIGN This is a single-centre, open-label, randomized controlled trial involving two parallel groups of female patients comparing a perioperative infusion regime wit...

A method is described for the isolation of subcellular membranes of Entamoeba invadens. Plasma membranes were obtained by rate centrifugation followed by isopycnic centrifugation on a sucrose gradient. Intact phagolysosomes floated in a 10% sucrose solution providing a simple technique for isolation. Phagolysosomal membranes were collected by isopycnic centrifugation, after lysis of the phagoly...

Present review article aims to explain use of injectable hydrogels and microspheres derived from natural polysaccharides as drug delivery systems and cell scaffolds. Polysaccharides isolated from natural sources are proved much better than synthetic polymers for making single and composite hydrogels. This paper emphasizes recent developments occurred in use of amphiphilic polysaccharides for bi...

The effects of acriflavine on the fine structure and function of the mitochondria and the kinetoplast in Crithidia fasciculata have been investigated. A mitochondrial fraction was prepared by differential centrifugation of cells broken by grinding with neutral alumina. Isolated mitochondria or intact cells revealed by spectrophotometric measurements the presence of cytochromes a + a(3), b, c(55...

Incubation of Ehrlich Lettréascites tumor cells with normal human serum at 37°C\vas found to result in the loss of a high percentage of cellular acid-soluble ribonucleotides, a decrease in lactic acid production, and a lower rate of endogenous respiration than in controls. Oxidation of succinate, a-glycerophosphate, and a-ketoglutarate was stimulated in cells preincubated in nor mal human ser...

Study of the activities of hyaluronidase, lactate dehydrogenase isoenzyme-X and the dehydrogenases of sorbitol, alpha-glycerophosphate, glucose-6-phosphate, malate, glyceraldehyde-3-phosphate and isocitrate in the testes of mice of different ages showed that the changes were correlated with the stages of spermatogenic cell differentiation. The first 4 enzymes had activities which were low in ne...