This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. For this purpose, Candida methylica FDH was covalently immobilized onto Immobead 150 support (FDHI150), Immobead 150 support modified with ethylenediamine and then activated w...

Human leukocytes in suspension or in monolayer cultures have been processed for electron microscopy by fixation in a freshly made cold mixture of glutaraldehyde and osmium tetroxide and by "postfixation" in uranyl acetate. Simultaneous exposure to glutaraldehyde and osmium tetroxide eliminates many of the shortcomings seen when either of these agents is used alone as the initial fixative. Speci...

An automatic microscope scanning and recognition system on base of the Utermöhl method is developed for image acquisition, archiving (optical fixation) and phytoplankton analysis. The system, called PLASA (PLAnkton Structure Analysis), enables a characterization of water specimens by their populations. It is described in detail with focus on the image analytical aspects. Plankton chambers are s...

A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H₂O₂ at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 - 8.5×10-5 M and ...

BACKGROUND This study was carried out to evaluate the effect of two commercially available chemical disinfectants namely sodium hypochlorite and glutaraldehyde and a surface wetting agent on the wettability of three high precision elastomeric impression materials, addition silicone, condensation silicone and polyether. MATERIALS AND METHODS Three different types of elastomeric impression mate...

This work presents a solution-processed gravure printed antenna on robust transparent nanopaper for potential Radio Frequency Identification (RFID) application. The nanopaper, having excellent dimensional stability in water, was obtained by glutaraldehyde treatment with hydrochloric (HCl) acid as a catalyst. For the first time, a device consisting of RF components for RFIDs was printed on stabl...

Protein hydrolysate (PH) was prepared from the defatted cottonseed cake by using immobilized or native trypsin on polyamide (nylon 6,6) matrix using glutaraldehyde coupling under the controlled conditions of pH and temperature. Immobilization of trypsin on nylon beads retained 30% of the free enzyme activity and was stable against leaching for more than 30 days. Foaming and emulsification prope...

OBJECTIVE Calcification is a frequent cause of the clinical failure of bioprosthetic heart valves fabricated from glutaraldehyde pretreated bovine pericardium. The major object of the present study is to prevent calcification of pericardial bioprosthetic heart valve materials with TPEN. METHODS Bovine pericardium was cut into 2-cm 2 pieces, rinsed in phosphate-buffered saline solution, transf...

Initial assembly of extracellular matrix occurs within a zone immediately adjacent to the chondrocyte cell surface termed the cell-associated or pericellular matrix. Assembly within the pericellular matrix compartment requires specific cell-matrix interactions to occur, that are mediated via membrane receptors. The focus of this study is to elucidate the mechanisms of assembly and retention of ...

PURPOSE Vinyl polyether silicone (VPES) has a different composition from other elastomeric impression materials as it combines vinyl polysiloxane (VPS) and polyether (PE). Therefore, it is important to study its properties and behavior under different test conditions. This study investigated the dimensional stability of 5 VPES consistencies when stored for up to 2 weeks, with and without using ...