The effect of temperature on umami taste has not been previously studied in humans. Reported here are 3 experiments in which umami taste was measured for monopotassium glutamate (MPG) and monosodium glutamate (MSG) at solution temperatures between 10 and 37 °C. Experiment 1 showed that for subjects sensitive to MPG on the tongue tip, 1) cooling reduced umami intensity whether sampled with the t...

Cytosolic carboxypeptidase 1 (CCP1) is a metallopeptidase that removes C-terminal and side-chain glutamates from tubulin. The Purkinje cell degeneration (pcd) mouse lacks CCP1 due to a mutation. Previously, elevated levels of peptides derived from cytosolic and mitochondrial proteins were found in adult pcd mouse brain, raising the possibility that CCP1 functions in the degradation of intracell...

1. [3H]Folic acid infused intravenously into patients with biliary fistulae appeared promptly in bile, coincident with 1311-labelled Rose Bengal injected simultaneously. The radioactivity was distributed among several fractions of biological folates present in bile and was associated with folic acid and with an unidentified folate which chromatographed on DEAE-Sephadex close to 1 0-formyltetrah...

Mitochondrial proton-translocating NADH:ubiquinone oxidoreductase (complex I) couples the transfer of two electrons from NADH to ubiquinone to the translocation of four protons across the mitochondrial inner membrane. Subunit PSST is the most likely carrier of iron-sulfur cluster N2 which has been proposed to play a crucial role in ubiquinone reduction and proton pumping. To explore the functio...

An enzyme, carboxypeptidase G1, extracted from a strain of Pseudomonas stutzeri, has been isolated which hydrolyzes the carboxyl-terminal glutamate from both reduced and nonreduced folate derivatives, with K, values between 1.1 and 18.1 X 10e6 M. This enzyme also hydrolyzes the COOH-terminal glutamate of oligopeptides and N-benzyloxycarbonyl glutamates; it demonstrates lesser activity against a...

Twarog, R. (University of Illinois, Urbana) and R. S. Wolfe. Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum. J. Bacteriol. 86:112-117. 1963.-A partially purified butyrokinase from Clostridium tetanomorphum has been found to phosphorylate valerate, butyrate, isobutyrate, and propionate. The divalent cation requirement is satisfied with magnesium. The pH optimum l...

The small multidrug resistance transporter EmrE is a homodimer that uses energy provided by the proton motive force to drive the efflux of drug substrates. The pKa values of its "active-site" residues--glutamate 14 (Glu14) from each subunit--must be poised around physiological pH values to efficiently couple proton import to drug export in vivo. To assess the protonation of EmrE, pH titrations ...

In contrast to methotrexate (MTX) and aminopterin (AMT), the 8-deaza analogues of these antifolates are not substrates for rabbit liver aldehyde oxidase. Since they are not converted to 7-hydroxy derivatives, they have been investigated with regard to their cytotoxicity for CCRF-CEM cells, transport into these cells, and conversion to polyglutamate forms. For this purpose 3H-labeled analogues w...

I read the recent systematic review which asked some interesting questions regarding the pathogenesis of tendinopathy (Vasta et al., 2016). The statement “histologic studies have demonstrated the absence of inflammatory infiltrates” is not supported by the current evidence base. Our recent systematic review on this subject demonstrated that the absence of neutrophils does not equate to the abse...