abundant in the spleen, the system we have described is absent from that organ in the mouse and has so far been found only in the liver. This finding agrees with the results of tissue-slice experiments. The stability of fl-glucuronidase to storage, to acetone precipitation and to armmonium sulphate fractionation is also in marked contrast with the above results. A purified spleen P-glucuronidas...

P-Glucuronidase has been purified from mouse kidneys previously induced by gonadotrophin to a specific enzyme activity 15 times higher than the non-induced kidney. The purification procedure includes ultrasonication to solubilize the enzyme, acid and ammonium sulfate precipitations, gel filtration in Sephadex G-200, DEAE-ion exchange chromatography, and isoelectric focusing. The resulting produ...

Hydrolysis of glucuronides is a prerequisite for the quantitative determination or the isolation of a number of physiologically important compounds which are conjugated with glucuronic acid in the animal body. The use of acid for this hydrolysis has frequently brought about chemical alteration of the product under study (14). The need for a hydrolytic agent which is specific and sufficiently mi...

The experiment was carried out on 20 Swiss male mice divided into experimental (E, n=10) and control (C, n=10) group. E mice were intraperitoneally injected with glucagon (15 μg/kg b.w.) twice daily for eight days, while mice C with 250 μl 0.9% NaCl/mouse (to exclude the injection effect only). In the lysosomal fraction of the liver the activities of acid phosphatase, β-N-acetyl-hexosaminidase,...