Rat liver glucokinase (EC 2.7.1.2) is inhibited specifically by the free form of palmitoyl-CoA (Ki apparent of approximately 1.8 PM) and other related long chain fatty acyl-CoAs under conditions where the micellar form of these compounds does not exist. This inhibition is instantaneous, and is immediately reversible by dilution, bovine serum albumin, and aor B-cyclodextrins. Inhibition is speci...

15 Phosphoenolypyruvate dependent glucose phosphorylation via the phosphotransferase system 16 (PTS) is the major path of glucose uptake in Corynebacterium glutamicum, but some growth from 17 glucose is retained in the absence of the PTS. The growth defect of a deletion mutant lacking the 18 general PTS component Hpr in glucose medium could be overcome by suppressor mutations 19 leading to high...

Supernatant fractions obtained by high-speed centrifugation of homogenates of liver and chemically induced primary and transplantable hepatomas were assayed kinetically, and by starch gel electrophoresis and column chromatography on di ethylaminoethyl (DEAE)-cellulose, for isoenzymes of glucose ATP phosphotransferase activity. Well and highly differenti ated hepatomas were similar to liver in h...