نتایج جستجو برای: glucoamylase i

تعداد نتایج: 1039473  

Journal: :Clinical chemistry 1978
R C Barabino D N Gray M H Keyes

We described a partitioned enzyme-sensor system, which incorporates an immoblized substrate and three or more discrete immobilized enzymes. This instrument measures alpha-amylase activity by passing the solution containing alpha-amylase over a column packed with immobilized starch. The resulting oligosaccharides are successively exposed to a column or columns containing immobolized glucose oxid...

Journal: :The Journal of general and applied microbiology 2018
Toshihiro Kojo Chihiro Kadooka Marisa Komohara Shiori Onitsuka Miya Tanimura Yukiko Muroi Shugo Kurazono Yohei Shiraishi Ken Oda Kazuhiro Iwashita Masahira Onoue Kayu Okutsu Yumiko Yoshizaki Kazunori Takamine Taiki Futagami Kazuki Mori Hisanori Tamaki

Aspergillus luchuensis is a kuro (black) koji fungus that has been used as a starch degrader for the awamori- and shochu-making industries in Japan. In this study, we investigated the effect of ion beam irradiation on A. luchuensis RIB2601 and obtained a high starch-degrading mutant strain U1. Strain U1 showed reduced growth rate, whereas it showed higher α-amylase, glucoamylase, and α-glucosid...

2016
Hui Zhang Shuang Wang Xiang xiang Zhang Wei Ji Fuping Song Yue Zhao Jie Li

BACKGROUND The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins i...

Journal: :Microbiology 2000
C L Gordon V Khalaj A F Ram D B Archer J L Brookman A P Trinci D J Jeenes J H Doonan B Wells P J Punt C A van den Hondel G D Robson

A glucoamylase::green fluorescent protein fusion (GLA::sGFP) was constructed which allows the green fluorescent protein to be used as an in vivo reporter of protein secretion in Aspergillus niger. Two secretory fusions were designed for secretion of GLA::sGFP which employed slightly different lengths of the glucoamylase protein (GLA499 and GLA514). Expression of GLA::sGFP revealed that fluoresc...

Journal: :Protein engineering 2000
H L Liu Y Doleyres P M Coutinho C Ford P J Reilly

Three single-residue mutations, Asp71-->Asn, Gln409-->Pro and Gly447-->Ser, two long-to-short loop replacement mutations, Gly23-Ala24-Asp25-Gly26-Ala27-Trp28- Val29-Ser30-->Asn-Pro-Pro (23-30 replacement) and Asp297-Ser298-Glu299-Ala300-Val301-->Ala-G ly-Ala (297-301 replacement) and one deletion mutation removing Glu439, Thr440 and Ser441 (Delta439-441), all based on amino acid sequence alignm...

2009
Nancy N. Nichols Bruce S. Dien Y. Victor Wu Michael A. Cotta

Cereal Chem. 82(5):554-558 Field peas (Pisum salivum) were evaluated as a potential feedstock for ethanol production. Ground peas were dry-milled and separated into starch, protein, and fibrous fractions by air classification. Starch-enriched fractions prepared from whole peas and dehulled peas contained 73.717c wt and 77.8% wt starch, respectively, a nearly two-fold enrichment compared with wh...

Journal: :Applied and environmental microbiology 2004
Marta Lombraña Francisco J Moralejo Rosa Pinto Juan F Martín

Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was observed between the level of the bi...

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