A (1→3)-β-D-Glucan produced by Lactobacillus suebicus CUPV221 strain was investigated by tapping mode atomic force microscopy (TM-AFM), to compare its supramolecular structure and conformation with two commercial polysaccharides: curdlan and scleroglucan. It was found that the β-D-Glucan was a (1→3)(1→2)-β-D-Glucan and at room temperature formed three-dimensional networks by entanglements betwe...

The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased...

We assessed the performance of galactomannan and (1→3)-β-d-glucan in 29 serum samples from patients with multiple myeloma and Waldenstrom's macroglobulinemia without invasive fungal disease to address issues of false positivity and uninterpretable results previously reported among patients with these conditions. Galactomannan and (1→3)-β-d-glucan assays were not falsely elevated in any patient....

Sucrose labeled in the fructosyl ((3)H) and glucosyl ((14)C) moieties was used to quantitate extracellular polysaccharide production and degradation by cariogenic and noncariogenic oral streptococci. All of the strains produced glucan and fructan. Streptococcus salivarius produced primarily fructan, whereas S. mutans and S. sanguis produced more glucan than fructan. The cariogenic streptococci ...

The yeastlike form of Paracoccidioides brasiliensis strain IVIC Pb9 reduced the amount of a-1,3-glucan in its cell wall from 45 to 3% when subcultured in vitro for several years. This strain regained its a-1,3-glucan up to 25% of the total cell wall when grown in vivo. A mutant strain of P. brasiliensis Pb9, named IVIC Pbl4O, reported to have 1,3-mannan instead of a-glucan in the cell wall, cou...

We assessed the effect of β-Glucan on macrophages by Griess reagent and viability by MTT assay and cytotoxicity. Assay of macrophages culture supernatants were carried out on WEHI-164 fibrosarcoma cell line as tumor necrosis factor-α bioassay were done. NO release was increased at the dose of 10 µg/ml (P = 0.001) of β-Glucan while the viability of macrophages in all concentrations was the same....

Kibite and Edney, 1998; Cervantes-Martinez et al., 2001). Cervantes-Martinez et al. (2001) conducted one cycle Oat (Avena sativa L.) -glucan lowers serum cholesterol in huof phenotypic selection of individual S0 plants for greater mans. Thus, enhancing its content in oat cultivars for human consumption is desirable. Phenotypic selection for greater -glucan content -glucan content in two genetic...

Beta-glucan is a polysaccharide in the form of fiber and the main element of fiber in grains such as barley, oats, yeast and mushrooms. Many studies have examined the efficacy of beta-glucan in terms of the lipid lowering effects, blood sugar reduction, weight reduction, immune modulator, and anticarcinogenic effect. However, there is no comprehensive review article on the biomedical issues reg...

Yeast cell wall matrix particles are composed entirely of beta-glucan and mannoprotein. Alkali-insoluble (1-->3)-beta-D-glucan was extracted from the yeast cell wall by an alkaline-acid method. IR spectra analysis showed that the product was chemically pure glucan, that is to say, it contained no other carbohydrates and proteins. So, the alkaline-acid method was ideal for extracting (1-->3)-bet...

The objective of the study was to evaluate the role of the mould cell wall agent β-glucan in environmentally related pulmonary disease. All published articles where β-glucan was administered by the airways, either as intratracheal injection or as inhalation were utilised as data sources. Data reporting consisted of analysis of data reports concerning the effects of β-glucan on the immune system...