in order to perform a histomorphometric study of blood cells in male adult ostrich, blood samples were obtained from jugular vein of 10 clinically healthy male adult ostriches (2 - 3 years old). the slides were stained with the giemsa methods and the smears were evaluated for cellular morphology, with cellular size being determined by micrometry. the findings of this study revealed that the sha...

Three methods of semiquantitative culture and two techniques of microscopy were compared with a surface viable count for the detection of significant bacteriuria in one thousand midstream specimens of urine. The results obtained with the blotting-paper-strip method on MacConkey agar and with Uricult dip-slides correlated well with the results of the surface viable count, and both methods were s...

In 2003, a 10-month-old Ghanaian boy recovered from a Trypanosoma brucei infection, although the patient was not treated with antitrypanosomal drugs. Only T. brucei gambiense and T. brucei rhodesiense are able to infect humans, causing human African trypanosomiasis. The disease is considered 100% fatal if left untreated. The identity of the trypanosome was determined by DNA extraction from the ...

This study concerns a comparative analysis of the acridine orange and Giemsa staining procedures for the fish erythrocyte micronucleus assay. The goal was to optimize the assay in the context of field water monitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l(-1) for 2, 4, and 6 days before testing. Slides from each individual w...

UNLABELLED Cat scratch disease, necrotizing granulomatous lymphadenitis caused by Bartonella henselae, usually benign and self-limited. However, various clinical manifestations and no pathognomonic histopathologic features can lead to misinterpretations and diagnostic disputes. We report a case of cat scratch disease in a 39-yr-old male patient with fever and left axillary lymphadenitis. He had...

they were washed with wet swabs and suspended in 1 ml. of nutrient broth and inoculated on blood agar media and into mice. All tested slides failed to produce either culture or infection. A similar experiment was performed with the same type of slides from which mercuric chloride was removed. Both the medium and mice failed to show the presence of B. anthracis. Because unstained areas round the...

BACKGROUND Immunohistochemical staining for p16INK4a and Ki-67 has been used to improve the accuracy in making a diagnosis of the uterine cervix cancer on biopsy. This study was conducted to examine the usefulness of these markers in the pathological diagnosis based on cervical biopsy. METHODS We selected a consecutive series of 111 colposcopically directed cervical punch biopsies. Using thes...

Many pathogenic fungi may be recognized grossly by their appearance in culture. The more definitive method of identification, however, is through microscopic examination of the structures on which classification depends: the spores and the sporebearing apparatus. The simplest method of accomplishing this is to tease a small fragment of mycelium out of the culture and crush it under a cover slip...

INTRODUCTION Extra-hepatic biliary atresia (EHBA) is an important cause of neonatal cholestasis. Several infectious agents have been proposed as etiologic factors such as Rotavirus and Reovirus. There is limited data on the role of Epstein Barr virus (EBV) infection in EHBA, so we decided to study the presence of EBV virus in a series of 16 proven EHBA cases by chromogenic in situ hybridization...

Helicobacter pylori is the most common gastric bacteria of human beings. Animal-borne helicobacter have been associated with gastritis, ulceration, and gastric mucosa-associated lymphoid-tissue lymphoma in people. We attempted to identify the species of Helicobacter spp. that infect human beings in north Paran , Brazil. Samples of gastric mucosa from 38 dyspeptic patients were analyzed by optic...