Background Circulating tumor cells (CTCs) could escape from the immune system through the programmed death-ligand 1 (PD-L1)/programmed cell death protein 1 (PD-1) axis leading to the development of metastasis. The current study investigated the expression of PD-1/PD-L1 on CTCs isolated from non-small cell lung cancer (NSCLC) patients treated with chemotherapy. Patients and methods CTCs were i...

Candida albicans cells which survive ingestion and multiply within phagocytes develop characteristic filamentous pseudogerm tubes. Candida cells killed by phagocytic leukocytes develop prominent alterations in Giemsa-staining characteristics; this reflects degradation of cyanophilic cytoplasmic components, probably ribonucleic acids. The numbers of these partially degraded organisms, termed "gh...

BACKGROUND AND OBJECTIVES In Iran, anaplasmosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare the detection of Anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional Giemsa staining method compared with the PCR-RFLP technique. MATE...

A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.

An exponential population of Saccharomyces cerevisiae cells was fractionated by centrifugal elutriation, using water as the elutriating liquid. Evidence that the population had been fractionated according to age in the cell cycle was obtained by examining the fractions for their size distribution, their microscopic appearance after Giemsa staining, and their ability to initiate synchronous growth.

BACKGROUND Prevalence of short segment Barrett's (SSB) oesophagus, defined as the absence of macroscopic Barrett's but histologically identifiable intestinal metaplasia, has been reported to be 18% based on haematoxylin and eosin (H&E) staining. AIMS To define the prevalence of SSB oesophagus using H&E and alcian blue staining and to determine whether SSB oesophagus is associated with inflamm...

The chromosomes of male and female individuals Enteromius callipterus [1] were assessed in this study with the objective determining diploid chromosome karyotype. samples obtained from Tahoss River, Jos Plateau, assessment was conducted using conventional Giemsa staining technique. reveals that individual E. has a number 2n = 50, fundamental autosomal arms (NFa) 82, karyotype formula 14M + 4m 3...

Recent work by Scliaudinn, Metchnikotf and others has directed attention to the spirochffite pallida as the probable causal organism of syphilis. Since cultures of this spirochaste have not yet been obtained by laboratory methods, its identification has largely rested upon the suitability of the staining reagents employed. Prominence has been given to Giemsa's method, which has already been rec...

Information on nuclei in bacterial spores was both scanty and inconsistent until the Feulgen technique, in the hands of Stille (1937), Delaporte (1939), Schaede (1939) and others, revealed Feulgen positive matter in the shape of a granule or rodlet at the periphery of the majority of mature, resting spores of many well known Bacilus species, including Bacllus megaterium. A more detailed picture...