Flow cytometry is frequently used in the evaluation of potential T-cell lineage lymphoproliferative disorders. Although flow cytometry is a useful tool, interpretation of the results can be challenging, because T-cells lack an easily analyzed structural element that can provide a surrogate marker of clonality such as immunoglobulin light chains on B-cells. Thus, routine T-cell phenotyping assay...

AIMS To analyse the cell cycle and DNA histogram components in data from DNA static cytometry and, in particular, to investigate the influence of the length of time the slides are exposed to the light of the cytophotometer in evaluating the G0/G1 peak. METHODS DNA static cytometry was performed on 18 Feulgen stained imprints and six histological sections taken from six breast carcinomas. The ...

Flow cytometry is a powerful, high-throughput, single-cell characterization and sorting tool, which has a unique capability to provide fast and quantitative analyses of individual cells and physical separation of cells of particular interest from other cells [1]. In flow cytometry, the cell analyses are performed by passing a narrow stream of cells through a focused laser beam at a rate of thou...

objective: immunotoxins (its) have been developed for the treatment of cancer, and comprise of antibodies linked to toxins. also vascular endothelial growth factor (vegf) plays a key role in tumor angiogenesis, and the blockade of vegf receptor-2 (vegfr2) inhibits angiogenesis and tumor growth. the aim of this study was to produce anti-vegfr2/rpe (pseudomonas exotoxin) 38 it to test its cytotox...

background: the poor permeability of the plasma and nuclear membranes to dna plasmids are two major barriers for the development of these therapeutic molecules. therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. objectives: the aim of this study was to investigate the in vitro delivery of plasmid dna encoding hpv16 e7 gene ...

Reduction in minimal residual disease, measured by real-time quantitative PCR or flow cytometry, predicts prognosis in childhood B-cell precursor acute lymphoblastic leukemia. We explored whether cells reported as minimal residual disease by flow cytometry represent the malignant clone harboring clone-specific genomic markers (53 follow-up bone marrow samples from 28 children with B-cell precur...