A proteolytic enzyme from L. muta stenophrys was isolated by gel filtration on Bio Gel P-100 followed by FPLC on MONO S column. The enzyme exhibited proteolytic activity toward casein, hemoglobin and fibrinogen with a pH optimum around 10. The activity was inhibited by EDTA while trypsin inhibitors were not inhibitory. It is a glycoprotein, Mr 14 kDa with a high content of Asp, Glu, and Leu res...

TiO2/Al-MCM-41 composites with various titania content were prepared by loading titania into the mesopores of Al-MCM-41 mesoporous molecular sieve from coal-measure kaolin as silicon and aluminum source via general sol-gel method and incipient wetness impregnation method. TiO2/Al-MCM-41 composites were characterized by XRD, FT-IR and SEM, and the photocatalytic degradation of methyl orange solu...

In order to obtain amino-functionalized silica materials with magnetic core, one-step synthesis was carried out. Several materials, differ in number and structure of amino groups, were synthesized on the basis of sol-gel method. The synthesized materials were examined by several analytical techniques. The presence and content of amino groups were measured by using Diffuse Reflectance Infrared F...

We have successfully synthesized relatively monodisperse and cross-linked melamine-formaldehyde (MF) microspheres by dispersed polycondensation and subsequent pH adjustment with serum replacement cleaning. The average particle sizes ( : weight-average and : number-average), the polydispersity index ( ), the number of particles Np, and the gel content of the M-F microspheres were observed by var...

Asphalt aging is one of the main causes asphalt pavement cracking, loosening and other issues. On a macro level, hardens becomes more brittle, while on micro chemical composition molecular weight distribution change. This very complicated physicochemical process. Gel permeation chromatography (GPC) powerful technical tool for understanding mechanism expressing GPC can be used to measure content...

Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between the three rDNA spacer types, the six pulsed-field gel types, and plasmid cont...

Abstract Rapeseed press cake was upcycled as a protein-enriched ingredient through dry fractionation. The fraction contained higher amounts of phytic acid compared to cake, and phytase treatment applied decrease the content from 6.8 0.5%. effect leading release cations also mimicked by extrinsic calcium addition. Both addition significantly improved heat-induced gel properties but had minor on ...