Activities and properties of the ammonium assimilation enzymes NADP+-dependent glutamate dehydrogenase (GDH), glutamate synthase (GOGAT) and glutamine synthetase (GS) were determined in batch and continuous cultures of Candida albicans. NADP+-dependent GDH activity showed allosteric kinetics, with an S0.5 for 2-oxoglutarate of 7.5 mM and an apparent Km for ammonium of 5.0 mM. GOGAT activity was...

A novel FAD-dependent glucose dehydrogenase (FAD-GDH) was found and its enzymatic property for glucose sensing was characterized. FAD-GDH oxidized glucose in the presence of some artificial electron acceptors, except for O2, and exhibited thermostability, high substrate specificity and a large Michaelis constant for glucose. FAD-GDH was applied to an amperometric glucose sensor with Fe(CN)6(3-)...

The aim of this study was to investigate the expression of glutamine metabolism-related proteins to determine whether glutamine is metabolized differently according to breast cancer molecular subtype. We generated a tissue microarray of 702 breast cancer patients and performed immunohistochemical staining for glutamine metabolism-related proteins, including glutaminase 1 (GLS1 (GLS)), glutamate...

The effect of salinity on the activity of glutamine synthetase (GS EC 6.3.1.2.) and glutamate dehydrogenase (NAD(P)H-GDH EC 1.4.1.2-4) in shoots and roots of triticale seedlings was investigated. Stressed plants were cultured hydroponically on the 100 mM NaCl-enriched medium for 7 days and the physiological responses were measured. Apart from changes in morphology of plants, alterations in GS a...

OBJECTIVE To assess the implications of falsely elevated glucose readings measured with glucose dehydrogenase pyrroloquinolinequinone (GDH-PQQ) test strips. RESEARCH DESIGN AND METHODS We conducted a review of the Food and Drug Administration's Manufacturer and User Facility Device Experience database and medical literature for adverse events (AEs) associated with falsely elevated glucose rea...

As first topic, the GDH sume rule is discussed in the context of a more general class of sum rules associated with the various contributions to the total photoabsorption cross section for target and beam polarization. Then I address the question of whether the GDH sum rule for the neutron can be determined from the one for the deuteron. It appears that this will not be possible in a simple mann...

The mutant strain am126 was isolated, using the direct selection procedure, after nitrous acid mutagenesis. It produced neither measurable NADP-dependent glutamate dehydrogenase (GDH) nor immunologically cross-reacting material. That the am126 strain produced some form of GDH product was shown by the fact that it complemented several other am mutant strains. The GDH formed by complementation be...

BACKGROUND Evidence regarding outcomes in the Geriatric Day Hospital (GDH) model of care has been largely inconclusive, possibly due to measurement issues. This prospective cohort study aims to determine whether treatment in a GDH could improve individualized outcome measures using goal attainment scaling (GAS) and whether improvements are maintained 6-months post-discharge. METHODS A total o...

Protein chemical studies of NAD(+)-dependent glutamate dehydrogenase (GDH; EC 1.4.1.2) from Clostridium symbiosum indicate only two cysteine residues/subunit, in good agreement with the gene sequence. Experiments with various thiol-modifying reagents reveal that in native clostridial GDH only one of these two cysteines is accessible for reaction. This residue does not react with iodoacetate, io...

New recommendations for testing and reporting of Clostridium difficile were introduced in the NHS in 2012. These guidelines have improved identification of potential C. difficile infection (CDI) cases, but questions remain around the management of glutamate dehydrogenase (GDH)-positive, toxin-negative patients. This study aims to assess the introduction of the Portrait C. difficile assay as the...