we have investigated the preventive effects of oral administration of isolated e.coli ghttam'tc acid decarboxylase (gad) in animal models. based on our results, the blood glucose levels were reduced by oral administration of gad to rats 14 days before intraperitoneal injections of streptozocin (40 mg/kg on five consecutive days). on the other hand, oral administration of gad to rats before stre...

IMPORTANCE High titers of autoantibodies to glutamic acid decarboxylase (GAD) are well documented in association with stiff person syndrome (SPS). Glutamic acid decarboxylase is the rate-limiting enzyme in the synthesis of γ-aminobutyric acid (GABA), and impaired function of GABAergic neurons has been implicated in the pathogenesis of SPS. Autoantibodies to GAD might be the causative agent or a...

Degeneration of CA3-pyramidal neurons in hippocampus after intracerebroventricular kainic acid (KA) administration, a model of temporal lobe epilepsy, results in hyperexcitability within both dentate gyrus and the CA1 subfield. It also leads to persistent reductions in hippocampal glutamate decarboxylase (GAD) interneuron numbers without diminution in Nissl-stained interneuron numbers, indicati...

OBJECTIVE To analyse the utilization of health care services of people who tested positive for GAD compared to those who tested negative. SETTING A cross-sectional study from the Northern Finland 1966 Birth Cohort. SUBJECTS A total of 10,282 members followed from birth in a longitudinal study were asked to participate in a follow-up survey at the age of 46. As part of this survey they fille...

Generalized anxiety disorder (GAD) is a common condition, with a lifetime prevalence of approximately 5% in the United States adult population according to the Epidemiological Catchment Area (ECA) study [1]. In comparison, Kendler et al [2] reported a lifetime prevalence of 3.6%. The National Comorbidity Survey reported a lifetime prevalence of 5.1% [3]. An international (World Health Organizat...

We have examined the distribution of neurons and terminals that are immunoreactive for glutamic acid decarboxylase (GAD), the synthesizing enzyme for the inhibitory neurotransmitter gamma-aminobutyric acid within the lateral geniculate nucleus of the cat. We estimate that GAD-positive neurons constitute approximately one-fourth of the neurons in all layers of the lateral geniculate nucleus and ...

OBJECTIVE The study of epileptic electroencephalograph (EEG) dynamics can potentially provide insights into seizure onset, evolution and termination. We propose a new synthetic measure based on time-frequency decomposition to provide detailed characterization of these dynamic changes. METHODS The matching pursuit (MP) method allows for continuous time-frequency decomposition. We have develope...

Objective: Patients with generalized anxiety disorder (GAD) are fearful. They constantly worried about minor matters, and they anticipate the worst. The GAD is diagnosed when a patient experiences excessive anxiety and worry for at least 6 months. The cause of GAD is unknown. In the present paper, we discuss patients with GAD who have low levels of thiamine in their bloods. We also discuss the ...

We have developed a gamma-aminobutyric acid (GABA) production technique using his-tag mediated immobilization of Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamate to GABA. The GAD was obtained at 1.43 g/L from GAD-overexpressed E. coli fermentation and consisted of 59.7% monomer, 29.2% dimer and 2.3% tetramer with a 97.6% soluble form o...