Two kinds of isoforms of glucose 6-phosphate dehydrogenase (G6PDH) were purified from cells of a freezing-tolerant strain, Chlorella vulgaris C-27, by sequential steps of chromatography on five kinds of columns, including a HiTrap Blue column which showed excellent separation of the isoforms from each other. The two isoforms (G6PDH1 and G6PDH2) were purified up to 109-fold and 197-fold with spe...

BACKGROUND Cyanobacteria are photosynthetic bacteria that thrive in diverse ecosystems and play major roles in the global carbon cycle. The abilities of cyanobacteria to fix atmospheric CO2 and to allocate the fixed carbons to chemicals and biofuels have attracted growing attentions as sustainable microbial cell factories. Better understanding of the activities of enzymes involved in the centra...

Soybean is an important legume food crop, and its seeds are rich in nutrients, providing humans animals with edible oil protein feed. However, soybean sensitive to water requirements, drought factor limiting yield quality. This study used Heinong 84 (drought resistant variety) Hefeng 46 (intermediate as tested varieties planted chernozem, albic, black soils. The effects of stress on the activit...

Metabolic Syndrome (MS) has become a major health hazard in the current world by triggering cardiovascular and renal pathologies. Chia seed is rich source of bioactive food components (n-3 fatty acids, antioxidants fiber, among others) with several benefits on health. Therefore, we hypothesized that intake chia could counteract kidney failure MS protecting against lipid accretion, oxidative str...

Malus baccata Borkh., an apple rootstock, is found to be damaged by oxidation at sub-low root-zone temperature. In previous studies, we have that exogenous sucrose could alleviate oxidative damage and increase the indole acetic acid (IAA) in roots under temperature (L). However, role of IAA sucrose-induced tolerance L remains unclear. A pot experiment was conducted evaluate effects synthesis/tr...

The Brilliant Cresyl Blue (BCB) test relies on G6PDH activity and a simple protocol for the selection of higher quality oocytes. Although the BCB+ oocytes of all the species that have been investigated are characterized by superior quality when compared to BCB- counterparts, application of the test for embryo production still remains an open issue. The aim of our study was to compare BCB+ and t...

BACKGROUND Acute kidney injury (AKI) is an important cause of preventable mortality among children. Management of AKI may require renal replacement therapy (RRT) but access to RRT for children in low resource settings is limited. Our study explored the role of haemodialysis in the management of children with AKI in a low resource setting in terms of aetiology and outcomes. METHODS A review of...

The origin of modern wheats involved alloploidization among related genomes. To determine if Aegilops speltoides was the donor of the B and G genomes in AABB and AAGG tetraploids, we used a 3-tiered approach. Using 70 amplified fragment length polymorphism (AFLP) loci, we sampled molecular diversity among 480 wheat lines from their natural habitats encompassing all S genome Aegilops, the putati...