The methanol inducible alcohol oxidase 1 promoter and the Saccharomyces cerevisiae alpha-factor prepro secretion signal were used to drive expression and secretion of a mucin-type fusion protein by Pichia pastoris in 1 L scale bioreactors. The aim of the study was to understand how varying expression rates influenced the secretion dynamics of the fusion protein in terms of intracellularand extr...

The homeobox genes are known to play a crucial role in controlling the development of multicellular organisms. The majority of these genes have been determined to express regulatory proteins act as a regulatory protein. These trans-acting factors regulate the expression of proteins that are necessary during the developmental processes throughout the body. TGIFLX/Y is a homeobox gene and it cont...

objective(s):to culture thein vitro mouse embryonic stem cells (mescs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct stra8-egfp to select differentiated es cells that entered meiosis.  retinoic acid was used to differentiate embryonic stem cells to germ cells. materials and methods: a fragment of stra8 gene pr...

Newcastle disease (ND), caused by the virus (NDV), is transmitted poultry with severe infectivity and a high fatality rate. The fusion (F) protein on NDV envelope facilitates merger of viral host cell membranes help homologous hemagglutinin-neuraminidase (HN). transmembrane (TM) domains proteins are typically required for fusion, but key amino acids in F TM have not been identified. Site-direct...

Newcastle disease, designated a class A disease of poultry by the Office international des epizooties (OIE), is an acute infection caused virus (NDV). The merging envelope NDV with membrane target host cell key step in pathway, which driven concerted action two glycoproteins: haemagglutinin-neuraminidase (HN) and fusion (F) protein. When HN protein binds to surface receptor, F activated mediate...

Drought is a major abiotic stress limiting agricultural crops production worldwide. In our study, we isolated novel C2H2-type zinc finger protein gene ZF2 from chickpea. consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 domain. Transient expression analysis ZF2:GFP fusion showed that was nuclear localized protein. the yeast assay system, full-length did not show transcriptional a...

background: dengue is a global arboviral threat to humans; causing 390 million infections per year. the availability of safe and effective tetravalent dengue vaccine is a global requirement to prevent epidemics, morbidity, and mortality associated with it. methods: five experimental groups (6 mice per group) each of 5-week-old balb/c mice were immunized with vaccine and placebo (empty plasmid) ...

lyme borreliosis is a tick-born disease caused by the spirochete borrelia burgdorferi. the aim of this research was molecular typing of different strains of b. burgdorferi and protective efficacy of outer surface protein in animal model. seven strains of b. burgdorferi isolated from skin and csf of lyme disease patients were compared using pcr- rflp as well as dna and amino acid sequences. the ...

So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...