The purpose of the present study was to test the possible plant thermotolerance role of isoprene and to study its relationship with non-enzymatic antioxidants and terpene emissions. The gas exchange, chlorophyll fluorescence, extent of photoand oxidative stress, leaf damage, mechanisms of photoand antioxidant protection, and terpene emission were measured in leaves of Quercus ilex seedlings exp...

A variety of diagnostic tests used by many laboratories to identify isolates of Wangiella dermatitidis (= Fonsecaea dermatitidis) were evaluated. Thirteeen isolates of W. dermatitidis were studied with respect to their ability to grow at 25, 37, 40, 45, and 50 degrees C, colonial and micromorphology, gelatin liquefaction, and hydrolysis of casein, xanthine, hypoxanthine, and tyrosine. All 13 is...

Much of the effect of heat stress on establishment and maintenance of pregnancy involves changes in ovarian function and embryonic development that reduce the competence of the oocyte to be fertilized and the resultant embryo to develop. There are three possible therapeutic approaches to manipulate the connection between hyperthermia and cellular responses to elevated temperature to improve fer...

27 To characterize the function of salicylic acid (SA) in acquired thermotolerance, the effects of heat 28 shock (HS) on wild-type and sid2 (for SA induction deficient 2) was investigated. After HS 29 treatment, the survival ratio of sid2 mutant was lower than that of wild-type. However, 30 pretreatment with hydrogen peroxide (H2O2) rescued the sid2 heat sensitivity. HsfA2 is a key 31 component...

In anticipation of using single or fractionated hyperthermia treatment in ex vivo purging of leukemic bone marrow in the clinic, we have compared the hyperthermic sensitivity, kinetics of thermotolerance, and heat-shock protein synthesis in three human myelogenous leukemic cell lines. In terms of heat sensitivity, the chronic myelogenous leukemic cell line K562 was found to be the most resistan...

Cullin4-RING ubiquitin ligase (CRL4) is a family of multi-subunit E3 ligases. To investigate the possible involvement of CRL4 in heat stress response, we screened T-DNA insertion mutants of putative CRL4 substrate receptors that exhibited altered patterns in response to heat stress. One of the mutants exhibited heat stress tolerance and was named heat stress tolerant DWD1 (htd1). Introduction o...

The growth and production of yeast in the industrial fermentation are seriously restrained by heat stress and exacerbated by heat induced oxidative stress. In this study, a novel synthetic biology approach was developed to globally boost the viability and production ability of S. cerevisiae at high temperature through rationally designing and combing heat shock protein (HSP) and superoxide dism...

Heat shock transcription factors (Hsfs) participate in a variety of plant physiological processes including the regulation associated with thermotolerance. Here, Hsf gene DcHsfA1d was identified from carnation (Dianthus caryophyllus L.). The open reading frame (ORF) 1 368 bp and encoded protein 455 amino acids molecular mass 51.039 kDa an isoelectric point 4.94. Sequence domain prediction revea...

In anticipation of using single or fractionated hyperthermia treatment in ex vivo purging of leukemic bone marrow in the clinic, we have compared the hyperthermic sensitivity, kinetics of thermotolerance, and heat-shock protein synthesis in three human myelogenous leukemic cell lines. In terms of heat sensitivity, the chronic myelogenous leukemic cell line K562 was found to be the most resistan...

Plants, as sessile organisms, employ multiple mechanisms to adapt to the seasonal and daily temperature fluctuations associated with their habitats. Here, we provide genetic and physiological evidence that the FtsH11 protease of Arabidopsis contributes to the overall tolerance of the plant to elevated temperatures. To identify the various mechanisms of thermotolerance in plants, we isolated a s...