To evaluate the effects of sequential, repetitive freezing on their in-vitro development, mouse embryos at the eight- to 16-cell stage were subjected to one of five treatments. They were (i) cultured as unfrozen controls, (ii) frozen once and cultured, (iii) subjected to two consecutive freeze-thaw cycles, (iv) frozen and thawed, and then cultured for 18-30 h before being frozen a second time, ...

Objective (s): The scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. The objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...

To improve bacterial removal, a traditional stormwater biofilter can be augmented with biochar, but it is unknown whether bacterial removal remains consistent as the biochar weathers during intermittent exposure to stormwater under dry-wet and freeze-thaw cycles. To examine the effect of weathering on bacterial removal capacity of biochar, we subjected biochar-augmented sand filters (or simplif...

The degradation of concrete material under multiple freeze–thaw cycles is an important issue for structures in cold and wet regions. This paper proposed a physical and mechanical model to explain the deformation behavior observed in previous experiments, from internal pressure calculation to mesoscale simulation, and for both closed and open freeze–thaw tests. Three kinds of internal pressures ...

In regions exposed to floods followed by cold weather, brick masonry as a structural basis of the building envelope can be damaged due accompanying phenomenon freezing and thawing. The main purpose article is development mathematical model able predict chosen mechanical parameters damage index wallets for given number freeze-thaw cycles. For this, statistical derived from experimental data used...