Watercress (Rorippa nasturtium-aquaticum), wild rocket (Diplotaxis tenuifolia), and spinach (Spinacia oleracea) are commercial crops reported to have high concentrations of antioxidants, possibly contributing to disease prevention following human consumption. Following analysis of supermarket-purchased salad leaves, we report the antioxidant content potential of these species using two comparab...

breast cancer is the second main cause of death among women. the use of medicinal plants has been common in many countries since ancient times. the aim of this study was to evaluate the antioxidant and anti-proliferative potential of achillea millefolium l. the antioxidant activity of the aqueous and hydroalcoholic extracts of achillea millefolium l. leaf and flower was measured by dpph and fra...

The microsomal enzyme uridine diphosphate (UDP) glucuronate glucuronyltransferase (E.C. 2.4.1.17) catalyzes formation of bilirubin mono-glucuronide from bilirubin and UDPglucuronic acid. Bilirubin glucuronoside glucuronosyltransferase (E.C. 2.4.1.95), an enzyme concentrated in plasma membrane-enriched fractions of rat liver, converts bilirubin monoglucuronide to bilirubin diglucuronide. Bilirub...

In recent years, data from fluorescence recovery after photobleaching (FRAP) has been analyzed quantitatively to estimate the binding rates of cellular proteins to cellular scaffolds such as chromatin. Unfortunately, the accuracy of these estimates is uncertain, since no benchmarks exist. To address this uncertainty, we have compared three different FRAP analysis procedures that have been appli...

Unconjugated bilirubin, conjugated bilirubin, and mixtures of various proportions of each were dissolved in depigmented human serum. These, together with normal and pathologic native human sera, were analyzed for conjugated, unconjugated, and total bilirubin by the Weber-Schalm method. Results were compared with the determination of “prompt direct reacting bilirubin” and of total bilirubin by t...

Both total and direct bilirubin measure the formation of azobilirubin, which is detected at 570/660 nm. The total bilirubin reaction contains surfactant and caffeine, which solubilize “indirect” bilirubin and accelerate the reaction (1). Direct bilirubin is a component of total bilirubin and therefore should always be smaller in value. The presence of a monoclonal immunoglobulin (2220mg/dL) fal...