Background: The aim of the present study was to develop a three-dimensional (3D) collagen gel culture system for the in vitro growth and survival of buffalo preantral follicles with or without growth factors. Materials and Methods: Buffalo ovaries were collected from a local abattoir and preantral follicles were isolated through microdissection. Isolated preantral follicles were put either in c...

The formation of urgent and long-term adaptation the organism under various modes motor loads is provided by a change in activity thyroid gland, functional unit which follicle. An accurate assessment morphological parameters follicles different histotopographic zones gland provides researcher with objective information about state organ. purpose study was to evaluate effect single training, sta...

The otter shrews are members of the subfamily Potamogalinae within the family Tenrecidae. No description of the ovaries of any member of this subfamily has been published previously. The lesser hedgehog tenrec, Echinops telfairi, is a member of the subfamily Tenrecinae of the same family and, although its ovaries have not been described, other members of this subfamily have been shown to have o...

There has been no culture system that supports the growth of bovine oocytes for more than 2 weeks. In the present study, bovine secondary follicles were cultured for 4 weeks, and the effects of supplemented protein components and FSH in the culture medium on the growth of the oocytes were examined. The effect of vitrification of secondary follicles on the subsequent oocyte growth was also exami...

objective: the purpose of the present study was to verify the ultrastructural characteristics of cultured mouse preantral follicles from fresh and vitrified –warmed ovarian tissues. materials and methods: the ovaries of 14-day-old mice (n = 5) were vitrified using a mixture of ethylene glycol, ficoll 70 and sucrose. the preantral follicles were isolated and cultured in α-minimum essential mediu...

BACKGROUND CD10 and CD34 have been detected in both epithelial and mesenchymal components of anagen human hair follicles. OBJECTIVES To analyse the expression of CD10 and CD34 in human hair follicle development as well as in different phases of the hair cycle. METHODS Fetal and adult hair follicles at different stages of the hair cycle were examined by immunohistochemistry for CD10 and CD34...

Ewes from selected lines of sheep from each of two breeds, Finns (high ovulation rate, low ovulation rate and control lines with respective ovulation rates of 5.4, 2.7 and 3.3) and Merinos (T Merinos selected for increased ovulation rate and control Merinos with respective ovulation rates of 1.9 and 1.2) were used to examine how selection to alter ovulation rate had altered follicle development...

Immature ovarian primordial follicles are essential for maintenance of the reproductive lifespan of female mammals. Recently, it was found that overactivation of the phosphatidylinositol 3-kinase (PI3K) signaling in oocytes of primordial follicles by an oocyte-specific deletion of Pten (phosphatase and tensin homolog deleted on chromosome ten), the gene encoding PI3K negative regulator PTEN, re...

In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicl...

Tumor necrosis factor-α (TNF-α) induces serum amyloid A (SAA) 3 among acute-phase proteins in mouse granulosa cells by activating NF-κB signaling via p55 TNF-α receptor type 1. However, the localization of SAA3 within the ovary is unknown. Here we investigated ovarian localization of SAA3 in a mouse ovulation model and in response to IL-1β, a proinflammatory mediator. For the ovulation model, e...