BACKGROUND/AIM To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. MATERIALS AND METHODS DNA was isolated...

results all 90 campylobacter isolates confirmed by m-pcr were successfully typed using flaa-pcr-rflp. eleven different types were defined according to flaa-typing method and the rflp patterns were located at three separate clusters in rflp image analysis dendrogram. conclusions campylobacter jejuni isolates significantly showed more variety than c. coli isolates. a relatively low genetic divers...

The adenosine A2a receptor (A2aAR) gene is thought to be involved in essential hypertension because adenosine elicits vasodilation and decreases arterial blood pressure via this receptor, and because disruption of the A2aAR gene increases blood pressure in mice. Therefore, using a restriction fragment length polymorphism (RFLP) of the A2aAR gene, we performed an association study in patients wi...

THO Complex 5 (THOC5) gene involves in lipid and fatty acid metabolism. The aim of this study was to analyse polymorphism THOC5 its association with composition sheep. A total 120 rams at the age 12 month average body weight 25–30 kg used for identification using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). 83 rams, representative from different genotypes shee...

This application note shows how the Agilent 2100 Bioanalyzer was used in polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) fragment analysis for fish species identification. A 464bp cytochrome-b target sequence, found in all vertebrate fish, was first amplified and then digested with restriction enzymes. The fragments were resolved on the DNA 500 LabChip®, allowing s...

Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberib...

Melanophilin (MLPH) gene affects dilution of coat colour. It focuses on D locus alleles and in c.-22G>A polymorphism 2 were described. Dominant allele controls regular colour whereas recessive d causes dilution. Diluted colouration Belgian Shepherd variety Malinois is undesirable excluded from breeding. Dilution the can sometimes be accompanied by skin disease (CDA or BHFD). ¬ Methods molecular...

Background/Aim: Alopecia areata (AA), which is characterized by hair loss, an inflammatory autoimmune disease. Tumor necrosis factor-alpha (TNFα) a potent proinflammatory cytokine that has highly significant role in and immune responses. The aim of this study to evaluate whether there relationship between TNFα -238 G/A gene polymorphism AA the Turkish population. Methods: In case-control study,...

This study aimed to detect the genetic association of HLA-G gene polymorphism with LSCC. It was a comparative estimating prevalence both 14-bp insertion versus deletion and HLA-G*01:05 N null allele using PCR-RFLP. highly recorded in patients LSCC (63.0%) whereas (37.0%) lower. The −14/-14 −14/+14 genotypes were significantly higher cases +14/+14 genotype not. found 39 (43.8%) 47 control (52.8%...

The world is going through the debate on hazardous effects of consumption of A1 milk on human health by producing of hazardous chemical compound like beta casomorphin-7 and how this is not the case with A2. The aim of the present study was to genotype the existing population of crossbred Holstein-Friesian (HF) cattle for polymorphism in â-Casein gene (CSN2) using PCR-RFLP technique. Total of 47...