The rate of glucose transport across the plasma membrane of the bloodstream form of Trypanosoma brucei was modulated by titration of the hexose transporter with the inhibitor phloretin, and the effect on the glycolytic flux was measured. A rapid glucose uptake assay was developed to measure the transport activity independently of the glycolytic flux. Phloretin proved a competitive inhibitor. Wh...

Kinetic model identification of metabolic networks is important, but still represents a significant challenge. The difficulties faced vary from the vast kinetic parameter search space to the lack of complete parameter identifiability. To meet these challenges, an incremental modeling approach is proposed here, including two key components—dynamic flux calculation and flux-based kinetic paramete...

Assessment of the relative importance of variation in enzyme concentration [E] and metabolic regulation in accounting for interspecific variation in metabolic rates is an unrealized area of research. Towards this end, we used metabolic flux rates during hovering and enzymatic flux capacities (V(max) values, equal to [E]xk(cat), where k(cat) is catalytic efficiency) in flight muscles measured in...

The polarity shift problem is amajor factor that affects classification performance ofmachinelearning-based sentiment analysis systems. In this paper, we propose a three-stage cascade model to address the polarity shift problem in the context of document-level sentiment classification. We first split each document into a set of subsentences and build a hybrid model that employs rules and statis...

This paper presents an automatic design method of an optimal inductive heating system modeled by finite element method. To obtain a uniform temperature distribution to the work piece surface, the inductor’s wrapping step is optimized by means of genetic algorithms. The 3D numerical model is provided by the Flux tools. The paper presents an innovative optimization procedure based on the scriptin...