A novel fluorimetric method for the determination of peroxynitrite (ONOO-) using hemoglobin (Hb) as a catalyst is described. The method employs the reaction of ONOO with thiamine (TM), a colorless, non-fluorescent reagent in a glycine-NaCl-NaOH buffer solution (pH 12.7), to generate a highly fluorescent product, thiochrome (TC). The fluorescent product was monitored by fluorimetry. A linear cal...

HE INCREASING USE of fluorimetry in routine analysis is reflected in a variety of commercially available instruments which, in many instances, are either prohibitive to the average laboratory due to high cost or lack proper sensitivity. Unlike the Beckman DU spectrophotometer, its clinical counterpart, known as Model B, is not provided with a standard fluorimetric attachment. The versatility of...

The samples from Xishanyao Formation in Gashunwusan Mine were analysed by inductively coupled plasma massspectrometry (ICP-MS), X-Ray fluorimetry (XRF) and other techniques. Through the use of the above techniques, we researched characteristics and modes of occurrence of the trace elements. According to content and ratios of trace elements, we discussed coalforming environments of the research ...

DETECHIP® is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit drugs for and over-the-counter medications, explosives, pesticides and food spoilage metabolites. DETECHIP® employs an array of sensors from which an analyte is tested and based on changes in color; a code specific to the analyte i...

HE INTRODUCTION OF the carboxylic acid cation exchange Amberlite XE-96 (or IRC-50) Azure A resin’ as the medium for hydrogen ion exchange in place of the quininium exchange resin has simplified greatly the determination of gastric acidity without intubation (1). Solvent extraction and fluorimetry are no longer required. The amount of blue dye excreted in the urine is readily measured by visual ...

Cys2His2 zinc finger proteins are important for living organisms, as they—among other functions—specifically recognise DNA when Zn(II) is coordinated to the proteins, stabilising their ??? secondary structure. Therefore, competition with metal ions may alter original function. Toxic such Cd(II) or Hg(II) might be especially dangerous because of similar chemical properties Zn(II). Most studies c...

Single-walled carbon nanotubes (SWCNTs) are a family of structurally related artificial nanomaterials with unusual properties and many potential applications. Most SWCNTs can emit spectrally narrow near-IR fluorescence at wavelengths that are characteristic of their precise diameter and chiral angle. Near-IR fluorimetry therefore offers a powerful approach for identifying the structural species...

The chemopreventive and anticancer effects of resveratrol (RSV) are widely reported in the literature. Specifically, mechanisms involving epigenetic regulation are promising targets to regulate tumor development. Bromodomains act as epigenetic readers by recognizing lysine acetylation on histone tails and boosting gene expression in order to regulate tissue-specific transcription. In this work,...

cAMP-dependent protein kinase (PKA) is an archetypal biological signaling module and a model for understanding the regulation of protein kinases. In the present study, we combine biochemistry with differential scanning fluorimetry (DSF) and ion mobility-mass spectrometry (IM-MS) to evaluate effects of phosphorylation and structure on the ligand binding, dynamics and stability of components of h...

Galectins are proteins involved in diverse cellular contexts due to their capacity to decipher and respond to the information encoded by β-galactoside sugars. In particular, human galectin-4, normally expressed in the healthy gastrointestinal tract, displays differential expression in cancerous tissues and is considered a potential drug target for liver and lung cancer. Galectin-4 is a tandem-r...