The so-called fluorescent group of Pseudomonas produces peptidic siderophores when grown in an iron deficient medium. They all contain (1S)5-amino-2,3-dihydro-8,9-dihydroxy-1 H-pyrimido[l,2a]-quinoline-l-carboxylic acid (7a) or its 5,6dihydro derivative as a chromophore. The 5-amino group is bound amidically to a small dicarboxylic acid (a member of the citric acid cycle) while the carboxylic g...

Phylogenetic relationships among a number of strains belonging to the genus Pseudomonas were explored by the use of in vitro deoxyribonucleic acid (DNA) hybridization. The fluorescent nomenspecies (P. fluorescens, P. putida, P. aeruginosa, P. cichorii, P. syringae, and related species), as well as the nonfluorescent species P. stutzeri, P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, we...

in this research, effect of vermicompost and plant growth promoting rhizobacteria (pgpr) on physiological traits of lemon balm under drought stress condition was investigated in year 2012 at karaj research institute of forest and rangelands. experiment was conducted as split-split plot in a randomized complete block design with three replications. irrigation (normal: irrigated to field capacity...

The phnA gene that encodes the carbon-phosphorus bond cleavage enzyme phosphonoacetate hydrolase is widely distributed in the environment, suggesting that its phosphonate substrate may play a significant role in biogeochemical phosphorus cycling. Surprisingly, however, no biogenic origin for phosphonoacetate has yet been established. To facilitate the search for its natural source we have const...

Five cosmid clones with insert sizes averaging 22.6 kilobases (kb) were isolated after complementation of 22 Tn5-induced Sid- mutants of Pseudomonas sp. strain M114. One of these plasmids (pMS639) was also shown to encode ferric-siderophore receptor and dissociation functions. The receptor gene was located on this plasmid since introduction of the plasmid into three wild-type fluorescent pseudo...

Whole genomic DNA-DNA hybridization has been a cornerstone of bacterial species determination but is not widely used because it is not easily implemented. We have developed a method based on random genome fragments and DNA microarray technology that overcomes the disadvantages of whole-genome DNA-DNA hybridization. Reference genomes of four fluorescent Pseudomonas species were fragmented, and 6...

Indigenous populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. that occur naturally in suppressive soils are an enormous resource for improving biological control of plant diseases. Over 300 isolates of 2,4-DAPG-producing fluorescent Pseudomonas spp. were isolated from the rhizosphere of pea plants grown in soils that had undergone pea or wheat monocultur...

The fluorescent emission of the thermolyzed Pseudomonas aeruginosa suspensions was measured in order to get a quantitative indicator upon the pyoverdine synthesis – as molecular basis of iron acquisition system of the bacterial cells. The influence of iron ions on the dynamics of pyoverdine was studied by supplying nano-sized iron oxide suspensions in the bacteria culture medium. The stimulator...

The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r produces several iron-regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant of WCS374r, its structure was determined following isoelectrofocusing and tan...

Bacteria that survive under variable conditions possess an assortment of genetic regulators to meet these challenges. The group IV or extracytoplasmic function (ECF) sigma factors regulate gene expression in response to specific environmental signals by altering the promoter specificity of RNA polymerase. We have undertaken a study of PvdS, a group IV sigma factor encoded by Pseudomonas syringa...